Measurement And Significance Of Helicobacter Pylori, Its Associated Protein And β-G In Bile And Mucosa In Patients With Gallstone

Objective: To study the relationship between the Helicobacter pylori(Hp) infection and the formation of gallstones.Methods: In this controlled study, the Hp DNA was detected in both 30 patients of gallstones( Experimental Group) and 10 patients of polypoid lesions of the gallbladder (Control Group) .Helicobacter pylori DNA in bile and mucosa of gallstones samples was detected by PCR,β-G activity was measured by using enzymological method. Meanwhile,Western-blot was performed to detect Helicobacter pylori associated protein in all bile samples.Results: The positive rate in experimental group of PCR amplification in bile and mucosa was 43.33% (13/30) and 53.33%(16/30) respectively, While only 1 positive in the control group of H. pylori DNA. The results showed that the activity ofβ-G significantly increased in experimental group as compared with controls, and there was bacterialβ-G activity no difference between the positive H. pylori DNA and the negative H. pylori DNA;but there was significant correlation endogenousβ-G activity in the positive H.pylori DNA Of gallstones. PCR showed positive in 13 bile samples,of which 9 contained anti-Cag A antibodies(69.23%),6 contained Vac A(46.15%),7 contained Ure B(53.85%),8 contained Ure A(61.54%). There were also Vacuolating cytotoxin associated 35kD and 30kD glycoprotein found in the bile. Also 1 samples of the positive H.pylori DNA in the control group contained anti-Cag A, Ure B, Ure A .While there is no associated protein of H. pylori infection in the negative of H pylori DNA .Conclusions: It was suggested that H.pylori DNA is present in bile and gallbladder mucosa from patients with gallstones. Infection of H.pylori in the biliary system may be one of the risk factors for gallstone formation and the infection may accelerate the formation of stones by enhancing the activity of endogenousβ-G activity or associated protein of H.pylori.