| Objective: To explore the biocompatibility of hydroxyapatite/silk fibroin (HA/SF)with bone marrow mesenchymal stem cells (BMSCs) and the osteogenic differentiation ofBMSCs in combination with several biomaterials, theoretically lending support to thepotential use of hydroxyapatite/silk fibroin(SF) materials as scaffold in bone tissueengineering.Methods: Four groups were included in the studies: Silk Fibroin (SF), collagen (C),hydroxyapatite (HA), HA/SF=1:1, HA/SF=4:1, tissue culture plastic. In order to observethe adhesion characteristic, growth and proliferation capability, an inverted microscope,hematoxylin and eosin (HE) staining and MTT test were used in this study. Flow cytometrywere applied to analyze the phenotype of BMSCs. Meanwhile, the BMSCs which culturedwith various materials were induced by conditioned medium containing Dex 10-8mol/L,β-GP 10mmol/L, and AA 50ug/ml. The properties of induced cells were measured byalkaline phosphatase (ALP) activity assay, modified alcium-cobalt staining, Von Kossastaining.Results: Compared with tissue culture plastic, SF, HA/SF=1:1, HA, and HA/SF=4:1materials can support the attachment, growth and proliferation. Especially, HA/SF=4:1can promote the attachment, growth and proliferation as does collagen. All materials testedhave no significant effects on phenotype of BMSCs. The induced cells have typicalosteogenesis characteristics. Results show that BMSCs grown on HA/SF=4:1 and HA have better osteogenesis ability than other groups, suggesting the unique characteristic of HA/SFin promoting the osteogenic differentiation of BMSCs and the potential use of thesematerials in bone tissue engineering.Conclusions: The HA/SF materials have good biocompatibility with bone marrowmesenchymal stem cells (BMSCs) and promote the osteogenic differentiation of BMSCs,suggesting that these materials could be the promising scaffolds in bone tissue engineering.
|
PDF Full Text Request