| The more and more evidence accumulated showed that transdifferentiation of endothelial cells to smooth muscle-like cells (TES) played an important role in the process of fibrosis diseases such as pulmonary or renal fibrosis and atherosclerosis. Costimulatory molecule CD40 message involved in the injure of endothelial cell's function. There remain unknown about CD40 signal in the role of TES. TGF-βwas thought to be the strongest factor participated in the epithelial-mesenchymal transition, whereas it is unclear about it's change in TES.Objective: To investigate the effect of costimulatory factor CD40 signal on TES and the role of transforming growth factor-[beta] in this process.Method: 1) The proliferation and apoptosis of HUVEC activated by 5C11 was measured with MTT and FCM. 2) Flk1 andα-SMA expression on the endothelial cell was detected by immuocytochemistry. 3) TGF-βreceptor was studied by use of FCM.Results: After endothelial cells were stimulated by 5C11, 1) Monoclone antibody 5C11 in this dose has little effect on the proliferation and apoptosis of HUVEC(P>0.05). 2) Decreasing Flk1 and increasingα-SMA expression were found, compare with control group(P<0.05). 3) High expression of TGF-βreceptor was detected.Conclusion: The expression of decreased Flk1 and increasedα-SMA after the stimulation of 5C11 show that CD40 message activation could induce the transdifferentiation of endothelial cells to smooth muscle-like cells. The effects of CD40 activation on the endothelial cell provide a new potential therapy target for the fibrosis related diseases. High TGF-βreceptor expression show TGF-βmessage may be involved in the process of TES by CD40 signal, which need further researched.
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