The Biocompatibility Study Of The H₂O Plasma-ion-implanted Pure Titanium

Part I Primary osteoblast culture and identification of SD ratPurpose To establish one ideal method of primary osteoblast culture and to supply osteoblasts for subsequent experiment;Methods In vitro incubate osteoblasts obtained from neotal SD rat calvaria by means of enzymatical digestion several times and then speculate cell morphology and assert the ALP activity;Results Primary osteoblasts were round-like,and then became triangular after 24-120 hour culture and mostly attached to the dish bottom;after 8-15 day,osteoblasts had grown monolayer;most of osteoblasts were ALP stained positively;Conclusions This method of primary osteoblast culture is feasible ,by which pure osteoblasts are made for the following tests.Part II The biocompatibility study of the H₂O plasma- ion- implanted pure titaniumPurpose To introduce one type of new H20 plasma- ion- implantation commercial pure titanium(cp Ti) and to preliminarily access the bioactivity of this material.Methods cp titanium which was inserted into the plasma ion implanter,under the control of fixed parameters,was implanted by water.To speculate the modified surface by AFM,and to test the surface chemical composition of it through XRD and FTIR.Osteoblasts obtained from SD rat clavaria were incubated in vitro under standard conditions and then seeded onto the modified surfaces at third passage.To take out of the samples at the 6th day of incubation,and then examine cell morphology and proliferation on the modified surfaces with the aid of SEM;untreated surfaces were regarded as control.Results The plasma-ion-implanted surfaces had been almostly completely covered by osteoblasts and abundant deposits which were closely compacted ,with large size, and pearl-like were deposited on these surfaces;cells had not so completely covered the control surfaces as the counterpart;Conclusions H₂O plasma-ion -implanted cp titanium has good biocompatibility and possesses bioactity due to probably promote deposition of Ca and P on those surfaces.Part III Comparative study of biocompatibility of H₂O plasma- ion- implantation commercial pure titanium and HA-coated titaniumPurpose To preliminarily compare the bioactivity of H20 plasma- ion- implantation commercial pure titanium(cp Ti) with the HA-coated titanium.Methods With the help of AFM and SEM,to individually speculate the surface topography of these two groups of modified materials.Osteoblasts obtained from SD rat clavaria were then seeded onto the two modified surfaces at third passage.To take out of the samples at the 6th day of incubation,and then examine cell morphology and proliferation on the modified surfaces with the aid of SEM;Results The plasma-ion-implanted surfaces had been covered by osteoblasts which extended comparatively well,and abundant deposits which were closely compacted ,with large size, and pearl-like were deposited on these surfaces;cells had also completely covered the HA-coated surfaces;what's more,the osteoblasts on this modified surfaces had bigger size ,more extensions,with cells overlapping;Conclusions It is likely that H₂O plasma-ion -implanted cp titanium has less biocompatibility than the HA-coated titanium,whereas it should be proven further;long-term results of cell culture need to be obtained by following tests.