Effects Of Growth Hormone And Basic Fibroblast Growth Factor On The Healing Of Ischemic Colonic Anastomoses In Rats

ObjectiveThe aim of this study was to evaluate the effects of growth hormone(GH)and the basic fibroblast growth factor (bFGF) on accelerating the ischemic colonic anastomosis healing in rats.Materials and methods100 adult male SD rats were divided into 5 groups (n = 20). Group A was the nonischemic control group. Group B was the ischemic control group.Group C was the ischemic control group receiving bFGF .Group D was the ischemic control group receiving GH. Groups E was the ischemic control group receiving bFGF and GH. Intraabdominal adhesions were scored,and bursting pressure and hydroxyproline levels were measured on the fourth and seventh postoperative days to evaluate anastomotic healing.And histologic examinations were also performed in each group.ResultsGH could increase both collagen deposition and bursting pressure significantly at postoperative Days 4 and 7 compared with the ischemic control group (P < 0.01),and it could decrease adhesion score significantly only at postoperative Days 4(P < 0.05). bFGF could also increase both collagen deposition and bursting pressure significantly at postoperative Days 4 and 7 compared with the ischemic control group (P < 0.01),and it could decrease adhesion score significantly at postoperative Days 4 and 7(P < 0.01) compared with the ischemic control group.The combination of GH and bFGF (group E) could also increase both collagen deposition and bursting pressure and decrease adhesion score significantly at postoperative Days 4 and 7(P < 0.01) compared with the ischemic control group,and when compared with the nonischemic control group ,it could increase both collagen deposition and bursting pressure and decrease adhesion score significantly at postoperative Days 4 and 7(P < 0.05).Histologic examination confirmed that bFGF and GH treatment both significantly increased collagen deposition and fibroblast ingrowth compared with controls.ConclusionsbFGF and GH can both significantly increase collagen deposition and bursting pressure and decrease adhesion score ,so they can enhance the ischemic colonic anastomosis healing and decrease the rate of colonic anastomotic leakage.