Study On The Effects Of Jianpi Yifei Huayu Recipe On Reversion Multidrug Resistance And The Gene Expression Of Lung Cancer

Objective: To Study on effects of Jianpi Yifei Huayu(JPYFHY) Recipe to inhibite the tumor of lung cancer and enhance the synergism of DDP, and reverse multidrug resistance of lung cancer chemotherapy, in order to provide objective experimental evidence for enhancing synergism of lung cancer in clinic.Methods: The model of Lewis lung cancer transplanted animals were applied to study the effects of tumor inhibition and synergism of enhancing the chemotherapeutic agents Cisplatinum(DDP). We take the lung cancer sensitivity cell A549 and resistance cell A549 / DDP for research object to observe the influnence cytotoxicity effects of JPYFHY Recipe drug-serum to the A549 and A549 / DDP, just as the cytotoxicity effects, and the influnence to cell cycle and the resistance reversion to DDP, which are determined by MTT assay. The expression of Multidrug resistance protein (MRP), Topoisomeraseâ…¡(Topoâ…¡)and Glutathione Stransferases(GST)ofA549 / DDP, are determined by Flow cytometry(FCM)respectively. At the same time, We observer the synergical reversion effects on drug resistance lung cancer when JPYFHY Recipe are combined with VPL in using.Result: The results show that JPYFHY Recipe had remarkably restraining effects, and it can significantly increase the inhibition rate of DDP to transplanted Lewis lung cancer. JPYFHY Recipe drug-serum has a certain cytotoxicities effects to A549 and A549 / DDP, and results in progressive increase in cells in G₁ phase and decrease in cells in S phase of the cell cycle. It can also significantly restrain the expression of MRP and GST, but enhance the activity of Topoâ…¡in multidrug resistance lung cancer cells. Lastly, JPYFHY Recipe and VPL both can reverse the drug resistance ofA549 / DDP, and the drugs can insert synergical effects when they are used in combination.Conclusions: JPYFHY Recipe can inhibite the tumor of lung cancer transplanted animals, and the JPYFHY Recipe drug-serum can block the prolife ration of A549/DDP in vitro by inducing cancer cell apoptoses and boking cell cycle; It can enhance the chemotherapy synergism effects when they are used in combination and reverse multidrug resistance which because of DDP. The mechanism of these reversing drug resistance maybe is related to downregulating the expression of MRP and GST and upregulating the expression of Topoâ…¡.