Pathological And Clinical Significance Of Co-detection Of TSG101, MDM2 And P53 In Lung Cancer Tissues

Objective To explore the pathological and clinical significance by co-detectingthe expression of TSG101, MDM2 and P53 in lung cancer tissue.Methods Formalin-fixed, paraffin-embedded tissues of 71 cases of lungcarcinoma were detected by immunohistochemistry assay using monoclonalantibodies to TSG101, MDM2 and P53. The results were analyzed by statisticalsoftware SPSS10.0.Results 1. The decreased expression of TSG101 protein was detected in77.14% of lung carcinoma cases, the overexpression of MDM2 protein was positivelydetected in 88.14% of the cases and 44.12% of the cases was positively detected forthe expression of P53 protein. 2. A positive correlation was shown between theexpression of TSG101 and MDM2 in P53~- group (P=0.004). There was no statisticsignificant difference between the expressions of them in P53~+ group. 3. A positivecorrelation was demonstrated between the overexpression of MDM2 and the grades ofthe studied lung carcinoma (P=0.001). The grades in both P53~-/MDM2~+ group andTSG101~-/ MDM2~+ group were relatively higher (P=0.005,P=0.005). Theexpression of MDM2 was positively related to the lymph node metastasis in lungcarcinoma (P=0.012).Conclusion 1. The decreased expression of TSG101 protein in the lungcarcinoma is associated with the formation of the carcinoma. 2. There is some controlloop among P53 protein, TSG101 protein and MDM2 protein in lung carcinoma. Apositive correlation between the expression of TSG101 protein and MDM2 proteinpresents in the lung carcinoma when P53 gene doesn't mutate. But when P53 genemutates, the positive correlation absents. 3. Co-detecting the expression of TSG101protein, MDM2 protein and P53 protein in lung carcinoma helps to judging the degreeof malignancy.