| Uterine cervix cancer is one of malignant tumors of genital system threatening women health and life, which may be resulted from socioeconomic factor, pathological changes of cervix, virus infection and reduction of immune function, and so on. The prevention and cure are difficult because of various pathogenesis. The therapy of uterine cervix cancer mostly uses chemosynthesis drugs at present, but side effects and multidrug resistance of chemosynthesis drugs become frequent in treatment. Naturally occurring drugs have been paid close attention by researcher for their low side-effect and no-drug-resistance. So screening naturally occurring drugs of anti-uterine cervix cancer becomes the study hotspot of pharmacology field.Fructus Arctii and Gleditsia sinensis sting were distributed widely in China. It is adjuvant of anticancer in common use used by herbalist doctor. The study on pharmacological activity of Gleditsia sinensis sting had not been developed widely yet. No study on anti-cancer activity of Fructus Arctii and Gleditsia sinensis sting was reported.In our study, we investigated anti-uterine cervix cancer activity of Fructus Arctii and Gleditsia sinensis sting by animal model of transplanted tumor cells. The results were as follows.(1) The effect of Fructus Arctii extract on the inhibition rate of solid tumor and the life span of ascites tumor were calculated by the animal tumor model experiment in vivo. In compare with the control group, the Fructus Arctii extracts (1000, 2000 and 4000 mg/kg body weight, p.o.) and CTX (25 mg/kg body weight, i.p.) administration significantly reduced the tumor weight of solid tumor and increased the life span of ascites tumor harboring mice (P<0.01). The inhibition rate of solid tumor and the rate of life span were up to 31.7,49.7,57.4,83.6% and 39.8,46.6,62.5,91.6% respectively. The studies suggest that Fructus Arctii extract has antitumor activity(2) The ethanolic extract from Gleditsia sinensis sting was prepared by hot refluence method. The effects of ethanolic extract on uterine cervix cancer harboring mice were studied. The results showed that it inhibited the solid tumor growth of solid tumor harboring mice and prolonged the survive of ascites tumor harboring mice. The tumor inhibition rate and the life prolong rate were 47.5%, 59.5%, 63.9% and 52.2%, 67.3%, 78.8% in low, middle and high ethanolic extract groups (250, 500 and 1000 mg/kg body weight). It showed that the ethanolic extract from Gleditsia sinensis sting had anti-uterine cervix cancer action in vivo significantly.(3) The study on anti-uterine cervix cancer activity of flavonoids showed that flavonoids (70, 140, 280 mg/kg body weight) inhibited the growth of uterine cervix cancer harboring mice. The tumor inhibition rate of solid tumor harboring mice and the life prolong rate of ascites tumor harboring mice were 44.2%,50.9%,54.6% and43.6%,47.9%,61.5% respectively. It indicated that flavonoids from Gleditsia sinensis sting significantly inhibited the growth of uterine cervix cancer-transplanted of mice.(4) With the methods of plant chemical constituent subentry test, we found that Gleditsia sinensis sting mainly consisted of saccharide, saponin, flavonoids, hydroxybenzene, protein, polypeptide, organic acid, lignin, steride, triterpene, essential oil and alkaloid, and didn't contain tannic acid, iridoid glycoside, quinone and terpene lactone.The study investigated anti-uterine cervix cancer effect of Fructus Arctii and Gleditsia sinensis sting systematically. It provided rationale to develop Fructus Arctii and Gleditsia sinensis sting as naturally occurring drug of anti-uterine cervix cancer.
|
PDF Full Text Request