| Objective: To investigate the effects of Xihuang pill drug serum on the growthand the cell cycle of breast carcinoma cell (MCF-7) and ovary carcinoma cell(SK-OV-3). Method: activity of tumor cell was detected by MIT and cell cycle wasanalyzed by flow cytometry. Result: Xihuang pill drug serum had an inhibitory effecton breast carcinoma cell (MCF-7) and, ovary carcinoma cell (SK-OV-3) which waspositively related with the drug content. Typital morphological changes of apoptosiswere observed under the transmission electron microscope. FCM showed that the cellcycle treated with Xihuang pill drug serum for 24h, the ration of cells in S phaseincreased, while that in G2/M phase decreased; after 48h treatment, the ratio of cells inG0/1 phase increased, and S phase decreased. Based on these data, we can concludethat Xihuang pill drug serum can inhibit the growth of breast carcinoma cell(MCF-7)and its cell cycle in vitro.And the typital morphological changes of apoptosis were also observed under thetransmission electron microscope. Cell cycle analyzed by FCM indicated that 72hafter Xihuang pill serum treated, the ration of cells in S phase increased and that inG2/M phase decreased. The conclusion resulted from the data related above is thatXihuang pill serum inhibit the growth and cell cycle of ovary carcinomacell(SK-OV-3) in vitro.Cell cycle analyzed by FCM indicated that 72h after Xihuangpill serum treated, the ration of cells in S phase increased and that in G2/M phasedecreased. |
PDF Full Text Request