Observation And Discussion Of Effect Of Endostatin On Xenograft Tumor Of Calu-6 In Mice

Background:Recently, the incidence and mortality of lung cancer in whole worldcontinue to increase and be highest in all of malignancy. About 80% ofthem are no small cell lung cancer. And 50% of patients given the diagnosisof NSCLC belong to advanced disease. They usually have several metastasiswithout discovered by routine medical examination. Thus, the NSCLCrelapse quickly after operation. Now, chemotherapy plays a key role intreatment of advanced NSCLC. But, efficacy of chemotherapy is verylimited. The phenomenon of drug resistant in NSCLC is very common. So,the target drugs become the hotpoint of research domestically andinternationally. Endostatin is the most promising drug among them, manydoctors try to use it in clinic. But we know a litter about its michenismand estimating efficacy.Objective:Observing the growth of xenograft tumor of Calu-6 in mice and the changeof biological marker which reflect proliferation,metastasis,apoptosis,angiogenesis of tumor treated by different dose Endostatin. Investigatethe mechanism of Endostatin and establish associated testing with tissueand peripheral blood in order to put it in clinical work and kinesisobservation.Methods: Treated with different dose Endostatin on xenograft tumor of Calu-6in mice, then detect markers like followings:1. To examine tumor tissue. Such as Survivin,VEGF,COX-2,C-erbB2 byimmunohistochemistry.2. circulating endothelial cells in peripheral blood by flow cytometry;its content of RNA by RT-PCR,real-time PCR.Recult:After teated with Endostatin, the express of Survivin protein,C-erbB2protein,COX2 protein,VEGF protein and MVD descend consistent, and thisdegression is dose dependent and the CECs in peripheral blood are diminish,this decreasion is dose dependent. Positive correlation are foundbetweenits circulating endothelial cells and its mRNA, circulatingendothelial cells and immunohistochemistry marker.Conclusions:1. Endostatin can inhibit tumors' growth and diminish expression ofSurvivin protein,C-erbB2 protein,COX2 protein,VEGF protein andMVD.2. The CECs in peripheral blood are diminish, this decreasion is dosedependent. Positive correlation are found betweenits circulatingendothelial cells and its mRNA.