| Purpose: Although treating that the vicinity loses getting notable progress in going ahead clinically, the curative effect is not very satisfied. Whose cause depends on (1) nerve regenerates slowly (generally 1 mm/day). (2) Degeneration, necrosis in different degrees happened in close nerve cell. (3) Distant-end effectors organ, especially skeletal muscle, produce loss nerve changing namely the atrophy, degeneration even necrosis. In many cases, lose the skeletal muscle that nerve controls nonreversible degeneration happened right away already sometimes in not having got effective nerve control the front again, have no way to restore whose function even if nerve regenerates to skeletal muscle. Though the speed and degree delaying losing nerve amyotrophic is the key that whether the muscle resolving weight obtaining nerve controlling the day after tomorrow again. The surviving nerve is able to send out the nerve axis under the situation of contacting with central nervous system insufficiency dashing forward, nutrition loses nerve skeletal muscle. The growth depending on whose the secretion nerve nutrition factor to boost an axis dashing forward, makes target tissue easy to gain nutrition between then, cutting off end if with having the active nerve basic cell kiss and on are away from cutting off nerve's, thereby immense the time having decreased by target tissue losing nerve nutrition, the atrophy delaying losing nerve skeletal muscle. Nervi spinals is the nervi spinals initiation headquarter , root and the abdomen are composed of from the back, they are that spinal cord has a common boundary one section of short nerve fiber , are that nervous centrals fiber changes the part for outside cycle nerve fiber , is called center vicinity carryover area. Root ganglions namely are large amount concentrated sensory neuron of compatriot body. Root ganglion in what environment to preserve can increase the quantity of the surviving nerve body and cuts down the death, is the key that ganglion cuts down target muscle atrophy and boosts peripheral nerve injuringDRGs is that the brachial plexus injured patient dumped organization after being wounded, if being able to make use of whose above-mentioned characteristic, transplanted it after the person is plucked burning in nerve which being damaged entering muscle, will cut down amyotrophic greatly so, thereby has created fine premise condition to treat the peripheral nerve loses. The experiment intends to study the rats root ganglion transplanting, hyperplasia condition, observing whose growth transplanting the day after tomorrow under the environment in diversity and surviving, discuss whose feasibility transplanting in human body inner, be that the ganglion transplanting studying the rat and person carry root ganglion further enters the change and effect nerve is damaged thereby lay down basis.Methods: 1, ganglion transplanting: experiment animal 45 SD rats, weight 430-450g, male and female there is no limited on, each other time has 9 rats. Randomly divides into A, B, C three group, Group A after 1% Wu barbituo sodium anaesthetized the queen with every kilograms of weight 40 mg in abdominal cavities, get rid of hair on back and fix it in the operating table, After touch the last costa which connected the spine, under it the three vertebra gaps place is L4 L5 antisternum body surface aerial. cut it skin upward L4,L5 under sterile condition, separating hypo and integrity snagged L4,L5 crest. open lumbar vertebra board and lateral protrusion under the microscope in L4 , L5 intervertebral space with pliers, we can see the lower spinal cord of flaval ligament. Use micro spring to cut open flaval ligaments revealing spinal cord being careful about, It can be seen that root ganglion, expands to the company in the upper back of spinal cord to become sphere, micro spring scissors pluck L4 ganglion, Entire take out pair of L4 ganglion, placed it in hypogastria subcutaneous fat(the underbelly has no main artery and nerve) , after that ,sewing skin up. in the same way, selected B , C L4 ganglion, B put in the quadriceps femora's, the group C peels off being put in the quadriceps femora's too. 2,Observe index: (1)Nissl dyeing and observing under light microscope: Ganglion takes out after transplanting 3, 7, 14, 28, 35 days, Nissl dye after an hydrating and pexying, 6μm blade, observing the change of spinals cell transplanting in each position. (2) HE dyeing are observed under light microscope: mainly observing state ganglion collagenoblas proliferation and blood vessel regeneration in the center of root.(3) specimens after FCA reagent to deal with count necrosis rate.Result: Observing in general ganglion which buried under skin parceled with organization around has the obvious blood vessel Pan to assume the radicalized aggregation vicinity and indicates it has built hemi around. Which group buried in muscle is difficult to identify because of color is same with approaching. Observing under light microscope we can see the neuron of dorsal root ganglion is cellular necrosis with different level in three conditions. The three-day reaches a peak, falls off gradually hereafter. The large amount of nerve cell inline shape accumulates the border in ganglion. Transplant the subcutaneous group of fiberation obviously in center because of short of blood, only some nerve survived near border place. The muscle group cuts down its necrosis because of containing rich blood transporting the protection nerve basic being able to be effective, the border survival nerve basic number is therefore more, central area occasionally sees. With the time extension, three set of necrosis rate all fall off gradually, three form ganglion central authorities at present large amount of fibroblast appears, but the muscle group is especially obvious. The morphology changes which operated after three days can be seen that each group of ganglion cellular nucleuses disrupt, the cell swelling becomes deformed and dissolves, kytoplasm puts color unevenly, the cell form is respectively different, and have different size, Assume long protuberance of multiplicity and much protuberance, Nissl disappeared after 1 week. About 2 weeks reaches a peak. Placed in sub skin are more frequent than placed in muscle. With the time extension, the center of ganglion appears amount of fibroblast after 3 to 5 weeks. Misdistribution, the coloration are deeper, have a little phlegmonosis cell infiltration, The survive nerve cell anaplasia disappeared gradually, the nucleolus becomes notable, the Nissl appears once again, the group which put in muscle can be seen a few entire karyon nerve cell which accumulates inline shape, the kytoplasm diameter enriches and abundant.Conclusion: The experiment result explains that:Rich blood can efficacious prevent neurotic dissolving and destroy, cuts down the collagen oblast hyperplasia, thereby cuts down the effect of swallowing nerve cell. The ganglion's adventitious did not affect the blood of neuronal. Therefore has also no effect to whose survival. if make the nerve fit on the side of the ganglion which abandoning in clinical, should put it in rich blood muscle, thereby creates the good nerve regenerates condition.
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