Effects Of Propofol And Remifentanil On Apoptosis Of Rat Alveolar Cells After Ischemia Reperfusion Injury

Objective The experiment wi1l research the effect of propofol and remifentanil against the apoptosis of alveolar cells induced by ischemia reperfusion injury.Methods 126 male Wistar rats weighing 220-250g were randomly divided into 5groups:GroupA:control group(n=6,species were taken directly after anesthesia and thoracic incision.This group served as the pre-operative controls to the other groups),Group B:Sham group(n=30,suture was only placed in right hilus.The other operation progress and analysis of species were same to IR group),Group C: Ischemia-reperfusion group(n=30,according to time,It is further divided into 5 sub-groups stands for before deligation,30min after ischemis,1h,2h,4h after reperfusion),Group D:propofol treatment group(n=30,five sub-groups as groupC), Group E:remifentanil treatment group(n=30,five sub-groups as group C).We use the rat model of warm ischemia and repefusion in situ in single lung.After the establishment of the ischemia reperfusion in situ models,all rats will give pharmaceutical intervention.Before the ligation,after 30min of the ischemia and after 1h,2h,4h repectively of the reperfusion,We get the lung tissue and blood samples of the right atrium,then detect the partial pressure of blood oxygen, the wet weight/dry weight ratio of lung tissue and observe the configuration and ultrastructure of the tissue cells to confirm that the apoptosis happens and detect apoptosis of alveolar cells with the flow cytometer.Results Compared with shame group,Ischemia-reperfusion group showed decreased PaO₂ and elevated wet/dry ratio(P＜0.05).Apoptotic index of lung was elevated.Compared with Ischemia-reperfusion group,propofol treatment group and remifentanil treatment group showed elevated PaO₂ and decreased wet/dry ratio (P＜0.05).Apoptotic index of lung was decreased(P＜0.05).Conclusions In conclusion,compared with Ischemia-reperfusion group,propofol treatment group and remifentanil treatment group showed elevated PaO₂ and decreased wet/dry ratio and apoptotic index of lung was decreased.It is said that propofol and remifentanil have suppression to the apoptosis of alveolar cells induced by ischemia reperfusion injury.