The Possible Involvement Of Ulinastatin(UTI) In The Development Of Acute Lung Injury(ALI)

Objective To explore the possible involvement of ulinastatin(UTI)in the development of lipopolysacccharide(LPS)-induced acute lung injury(ALI).Methods We selected thirty male SD rats which have same condition,then have built three groups:control rats(being injected with normal sodium 1ml via caudal vein),LPS rats(being injected with LPS via caudal vein as ALI sample),LPS+UZI rats(being injected with UTI shortly after injected with LPS).We determined the concentration of interleukin-18(IL-18)in abdominal aorta and in lung tissue by ELISA from male SD rats 4h after injected with LPS.and determined the concentration of nuclear factor-κB(NF-κB)in lung tissue by immunohistochemistry.Then we got pathological section and electron microscope section of these rats lung.Results The IL-18 of abdominal aorta and lun tissue was significantly higher in the rats at 4h after LPS injected than in control rats P＜0.01,The level of IL-18 in LPS+UTI rats also little higher than in control rats,but there was not obvious difference between the two groups P＞0.05.The concentration of NF-κB in LPS rats was significantly higher than in control rats P＜0.01.The level of NF-κB in LPS+UTI rats was also little higher than in control rats,but there was no obvious difference between two groups.Pathological section and microscope section also supported that the inflammation reation of LPS+UTI rats lung is lighter than LPS rats'.Conclusion It is suggested that given UTI in ALI rats can play an important role that it canⅡdecrease inflammation reaction of ALI.