Mandible Bone Repair In Rabbits With LDIG/n-HA Composites

Objective: To observe the heal process of LDIG/n-HA compositesrestore the rabbits skull wane. Following observations and evaluations werecarried out of optimizing composites for bone regeneration. The mainlypurpose of this experiment was to give a preliminary experimentalevidence and to prove the novel composites as potential bone substitutionsor bone tissue engineering scaffold.Methods: 1. Preparation of materials: The materials were provided byNational Hepatobiliary & Enteric Surgery Research Center-National KeyLaboratory of Nanobiological Technology. After preparation, the physicaland chemical properties of the composites were preliminary studied.2.Animal experiments: 36 adult rabbits were divided into 3 time groupsrandomly: A: LDIG/n-HAP group;B:n-HA group; C:post-operative group.After2, 4, 8 and 12 weeks post-operation, the experimental rabbits analyzedby radiographic, following, the animals of every groupwere killedaccording to different operalion period and the implantations were taken offand studied by gross observation,X-ray density, histological analysis andX-ray gray extent of callus analysis.Results:①After 12 weeks post-operation,the LDIG/n-HAcomposites group can restore the wane completely, and every period X-rayestimate all good than the others, and it have an exponentialcorrelation(P＜0.05).②n-HA group have no exponential correlation inevery period(P＞0.05),and at 12th week its restore effect is worst than Agroup(P＜0.05).③The bending strength of callus and the X-ray density ofcallus have an exponential correlation(P＜0.05).The X-ray density of callus increased coincidently with the fracture healing process. It also confirmedthat there was a high correlation between the character of bending strengthof callus and the X-ray density of callus.Conclusions: The LDIG/n-HA composites have a strong power ofcoordination with mature bone, and it has very good biocompatibility andbiodegradability. It has some power of bone guide and bone transmits, itcan replace homograft bone, and they were suitable for the growth ofosteoblasts and new little blood vessels as well as reformation of new bonetissues.