Study Of Wogonoside Inhibitor On Human Liver Cancer Cell Line BEL-7402

The cancer of the liver is one of the familiar malignant tumors of our country, difficult to be treatment in clinic. It has been a hotspot to control the gene related to the cell aopoptosis and induce the cell aopoptosis in research of tumor treatment recently. The anti-tumor function of the flavonoid in Radix scutellariaeis expresses actively in vivo and in vitro. But current research domestic about wogonoside in vitro is poor. Our studies of wogonoside inhibitor on human liver cancer cell line Bel-7402 aims at discussing the mechanism of wogonoside inhibitor of tumor and providing theories for pharmaprojecting medicine of wogonoside clinical application.We treated the cultured Bel-7402 cell with wogonoside on different density; observe the change of cell appearance through microscope observation; and the cell stained with AO/EB through the fluorescence microscope. We discover that the wogonoside can repress the cell Bel-7402 growth obviously, the fluorescence microscope show clearly cell apoptosis. We cultured cell in 96 bores board, examines the absorbance on 570nm after MTT experiment. Cells proliferation was inhibited by wogonoside density above 4μM. The 50%-inhibitory concentration (IC₅₀) was 8μM for 48hours respectively. DNA fragmentation was visualized by agarose gel electrophoresis, DNA Agarose gel electrophoresis of the treated cell revealed "ladder" pattern. All above shows that wogonoside can inhibit cell proliferation of Bel-7402 and induce cell apoptosis.For studding the mechanism that the wogonoside repress the cells Bel-7402s and induce it apoptosis, cell apoptosis rate and cell cycle arrest was detected by flow cytometry. The flow cytometry profiles revealed that wogonoside induced Bel-7402 cell G₂/M arresting because the reduce of G₀/G₁ rate and the increase of S and G₂/M in 60hrs. It also show that the rate of apoptosis cell was increase when the density was increased (4～20μM) during the same time. Expression of Bax/Bcl-2 and P53 was analysed through Western blotting. Wogonoside induced the down-expression of Bcl-2, and the increase of Bax/Bcl-2 ratio through Western blotting. It had no effect on expression of P53.In conclusion wogonoside can inhibit cell proliferation of Bel-7402 and induce cell apoptosis. It may be related with the increase of Bax/Bcl-2 ratio but not P53 through the mitochondria pathway.