Experimental Research On Sodium Alginate Involved Medical Adhesive In Bone Block Fixation

Objective: To verify the medical adhesive which is mainly composed of sodium alginategels can be a new adhesive to fix small bone block, by which we undertook in vitro and animalexperiment researches on bone block fixation.Methods:1 In vitro experiment: Cortex bone of femur from healthy pigs were cut into rectanglebone blocks and fixed by the glue, and the shear stress force of the bone blocks weremeasured that day and the days 1,2,3 weeks after saline infiltrated.2 animal experiment: 65 mature New Zealand rabbits were randomly assigned intocontrol group,experimental group and blank group. Similar-sized bone blocks were takenfrom left radius in all three groups. The bone blocks were fixed with glue in experimentalgroup, fixed with No.4 thread in control group, and without any fixation in blank group.Theyare fed after organization sewn up. Peniciline was used for anti-infection postoperatively.Blood biochemistry arrays were undertaken 3,7,14 days postoperatively. Radiography ofipsilateral limbs was taken 1,2,4,6,8 weeks respectively to observe the bony callus growth.All animals were sacrificed in batch and histological sections were observed through lightmicroscope and electron microscope.Results:1 In vitro experiment showed that this adhesive could firmly fix bone blocks whosesection is about 1cm~2 size.2 Animal experiments showed that blood biochemistry index including cardiac,hepaticand renal function, main electrolytes and ALP(alkaline phosphatase) in experimental grouphave no significant differences compared with those in control group. Radiography determination showed that there were 5 significant migration in blank group, 4 inexperimental group and bone healing time was equivalent among experimental group andcontrol group. There were pinhole like spaces within bone callus in experimental group andthe quality of callus was not good enough. Histology and ultrastructural observation showedthat there were glue residuals and small amounts of inflammatory cell infiltration in localtissues in experimental group 2 weeks postoperatively. Bone callus formation was slightlyslower than that in control group in earlier stage. There was no significant cytotoxicity amongcallus ultrastructure in experimental group.Conclusions:1 The animal model could basically meet the experimental request and be expected tobecome a standard model of such preliminary experiment on bone blocks.2 The experimental glue has good biocompatibility, no significant toxin side effect inshort time and no effct of delaying fracture healing.3 Sodium alginate and SCMC mixture with calcium chloride could effectively fix boneblocks of a certain size and could be a new material of pasting bone blocks.