The Expressive Study Of MAPK In Human Cholangiocarcinoma

ObjectiveThe attack rate of cholangiocarcinoma increases fastest in the carcinoma of peptic tract, it is the second carcinoma in liver and gallbladder system except hepatocarcinoma. The same to other malignant tumor, the development of cholangiocarcinoma due to many factors, it is a complex process, the mechanism is unknown now and there is no effect diagnosis and treatment for this. So, it is very important to find tumor-marker, treatment-marker and mechanism of onset.The development of malignant tumor refers to proliferation, apoptosis and transformation. That pathological mechanism has relationship with regulation of signal Conductive pathway. Mitogen-antivated protein kinase is a kind of serine and threonine residual group protein kinase, and a important family of extracellular signal transduction enzyme. The development process refering to multi-layer regulation of cell can transfer many extracellular signal to the nucleus through activation of phosphorylation. MAPK includes three main important sub-family, extracellular signal-regulated protein kinase, c-Jun and p38. They are activated by special enzymes, MAPK signal transduction pathway involves three kinds of protease which are activated in sequences, one is the kinase of MAPK kinase, one is MAPK kinase and the last one is MAPK. MAPKs can be phosphorylation activated by special MEK in threonine and tyrosine double site, it is also phosphorylation activated by special MEKK in threonine and serine double site. Each kinds of MEK can be activated by at least one kind of MEKK, each kinds of MAPK also can be activated by different MEK, it sets up MAPK complex regulation network. MAPKs can stay in cytoplasm after activated, activate a series of others protease, make cellular skeleton phosphorylation, promote synthesis of relative protein and changing of canal, and finish reaction of extracellular stimulation.This experiment collects 62 surgery samples of cholangiocarcinoma, the section embedded in wax. The analysis of expressive difference about ERK1, JNK1 and p38 in the tissue of cholangiocarcinoma is through immuno-method.MethodThe pathological samples are from 62 cholangiocarcinoma surgical tissue of the first affiliated hospital of CMU from Jan of 1999 to Dec of 2005. We detected the expression of ERK1, JNK1 and p38 protein in the cholangiocarcinoma tissue by immunologic histochemistry methods. Statistic analysis was completed with statistic software SPSS 11.0.Results1 ERK1, JNK1 and p38 positive staining is main in nucleus (picture), but a little expression in cytoplasm. In 62 patients, low differentiation in ERK1, JNK1 and p38 is 28(45.2%), 40(64.5%) and 36(58.1%) respectively, and high differentiation is 34(54.8%), 22(35.5%) and 26(41.9%) respectively.2. JNK1 and p38 is negative related to pathological degree. (r=-0.350, P=0.005, r=-0.300, P=0.018), in high differentiated cholangiocarcinoma tissue, the content of JNK1 and p38 is more than that of low differentiated group. The protein expression of ERK1 is positive related to pathological degree(r=0.436, P<0.0005). The content of ERK1 protein in low differentiated cholangiocarcinoma is obvious higher than that of high differentiated group. But all are no relation to age and TNM.3. The expression of ERK1 protein is negative related to p38 (r=-0.345, P =0.006), and there is statistic meaning. There is obvious positive relation between the expression of JNK1 protein and that of p38 protein, it has stastic meaning. Then there is obvious negative relation between the expression of ERK1 protein and JNKl(r=-0.140, P=0.278), but no stastic meaning exists.Conclusions1 ERK1 protein has close relationship with pathological differentiated degrees in cholangiocaicinoma tissue, the letter differenciation, the more activated proliferation and the higher expression of ERK1 protein.2 The expression of JNK1 and p38 protein in high differentiation cholangiocarcinoma tissue is higher than that of low differentiation cholangiocarcinoma tissue. May be the expression of JNK1 and p38 protein is a protective factor, but now no enough proof can prove this. It is needed a lot of sample for the proof of this3 ERK1, JNK1 and p38 have a certain relation in protein level, but it is needed further study for its biological significance. In a word, the expression of MAPK family protein may be related with the being and development of cholangiocarcinoma.