Protective Effects Of Penehyclidine Hydrochloride On Liver Injury Of Rats Induced By Cardiopulmonary Bypass

ObjectiveTo investigate the protective effects of the Penehyclidine Hydrochloride on liver injury of rats induced by cardiopulmonary bypass and the mechanisms involved.Methods32 health male SD rats weighing 300-450g were randomly divided into 4 groups of 8 animals each:(I) Sham; (II) Mod; (III) L-PHC; (IV) H-PHC. The general anaesthesia of animals were performed with chloralic hydras(10%, 0.3ml/100g) ip. To maintain the depth of anesthesia with chloralic hydras during operation. Group I only received arteriopuncture and veinpuncture without CPB. Group II, Group III and Group IV were monitored sustainedly during CPB including heart rate, mean artery pressure, anus temperature, respiration rate, arterial blood gas test value(Group I was also monitored with the same process). Penehyclidine Hydrochloride which group III(0.6mg/kg) and group IV(2mg/kg) were added to priming solution of CPB. When the temperature of rectum reached about 37.0℃, stopped CPB. CPB was maintained for 60min. Blood samples were taken at the end of CPB for determination of serum activities of alanine am inotransferase (ALT) and aspartateam Inotransferase (AST). Then the animals were sacrificed for obtaining liver specimen. The morphological changes in the liver cells and liver cell devices were observed under the electron microscope(H-7200).ResultsSerum ALT and AST activities were significantly higher in group II, group III and group IV than in group I (P<0.05 or P<0.01). In comparison with group II, group III and group IV, the levels of Serum ALT and AST activities respectively were Mod> L-PHOH-PHC(P<0.05 or P<0.01). The electron microscopic examination showed that the pathologic changes were serious in group II compared with group III and group IV. Meanwhile, in comparison with group III and group IV, the pathologic changes were slight in group IV.ConclusionThe Penehyclidine Hydrochloride can protect liver of rats against injury induced by cardiopulmonary bypass. The effect is significant in group IV.