Research Of Chitosan's Improving Proliferation Effect To The Bladder Epithelial Cells

Objective To study chitosan's improving proliferation effect to the bladder epithelial cells,thus providing experimental foundation for the treatment of interstitial cystitis.Methods Bladder epithelial cells were harested by the enzymatic digestion of the epithelium exposed by the eversion of resected New-Zealand ha- re's bladder; The effects of chitosan on proliferation of rabbit bladder epithelial cells were studied by NAG assay and cell counting; Cell specificity was confirmed by immunohistochemical staining. EGFR mRNA was measured by PT-PCR. Result The growth of cells in the sample added chitosan is much better than that of in the control group; Chitosan could promote the proliferation of bladder epithelial cells at higher than 0.03 % of concentration in a dose dependent way. The optimum concentration to increase proliferation of conjunctival epithelial cells was 0.12 %. Immunohistochemistry revealed that keratin staining was positive. The proliferative effect of EGFR mRNA increased with the elevated chitosan concentration after 72 h. Conclusion Chitosan can promote the growth of the bladder epithelial cell, which can provides a valuable evidence for further studies of interstitial cystitis's treatment. This proliferation effect is perhaps related to chitosan's promoting EGFR combinating specificity with EGFR.