| Objective To investigate the effect of inducing differentiation on human gastric cancercell line SGC-7901 with arsenic trioxide and to approach its potential mechanisms.Methods SGC-7901 cells were treated with arsenic trioxide(As2O3) at the concentrationof 0.5μmol/L, for 24, 48, 72h respectively. Cell growth and proliferation were observed bycell counting and trypan-blue-exclusion. Morphologic changes were observed with lightmicroscopy. Flow cytometry(FCM) was used to assay apoptosis and cell cycledistribution. The expression of Bcl-2 and P53 was examined by immunohisto- chemistry,method.Results①After constant exposure to 0.5μmol/L As2O3 for 24, 48, 72h, the growth ofSGC-7901 cells was markedly inhibited, which was confirmed by cell counting.The timeof cell double in amount prolonged from 70.7h to 125.0h at the 5th passagetime(P＜0.01, compared with that of control group).②FCM showed the apoptotic brugafter exposure to As2O3 72h, but the number of apoptotic Cells was minor(5.54%), nostatistically significant compared with that of control group(0.81%).③The evidentchanges of cell cycle induced by As2O3 in lower concentration was shown by FCM: theproportion of G0/G1 phase cells increased after treated whith As2O3 and the S phase cellsdecreased(48h: P＜0.05, 72h: P＜0.01, compared with that of control group respectively).④After three days of culture with As2O3, the expression of Bcl-2and P53 decreased incomparison with that of control group(P＜0.05, P＜0.01).Conclusions①0.5μmol/L As2O3 can markedly inhibit cell proliferation of humangastric cancer cell line SGC-7901 through the induction of cell differentiation in vitro.②As2O3 may be an effective inducing-cell- differentiation drug and an potentialanti-gastric-cancer agent. It deserves further research.
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