| Objective Observing the effect of radices salviae miltiorrhizae combining with intrarenoushyperoxic solution on healing bone coloboma of rabbits using histological andbiochemistry method. Studying the therapeutic effect of radices salviae miltiorrhizaecombining with intrarenous hyperoxic solution on bone coloboma healing of rabbits.Methods 24 rabbits are randomly divided into four groups after bone coloboma modelbuilding, the radices salviae miltiorrhizae treated group (R group, n=6), the hyperoxicsolution treated group, (H group, n=6), the radices salviae miltiorrhizae and hyperoxicsolution group(the compound group, C group, n=6) and the saline control group(Sgroup, n=6).(1) At the 1st,14th and 28th day of treatment, the growth of callus wereobserved by the X-ray; (2) At the 14th and 28th day of treatment, the callus tissue isobserved by Hematoxylin-Eosin(HE)staining; (3)At the 14th and 28th day, the content ofbasic fibroblast growth factor(bFGF) of the callus tissue is observed byimmuno-histochemistry assay;(4)At the 287th day, the hydroxyproline(HYP) content of thecallus tissue is observed by defluonnated water solution. Data was analyzed statisticaly.Results (1) C group could increase the levels of osteoblast of callus, which wassignificantly higher than other groups;(2)The bFGF content of callus tissue had beenobviously improved in C group than other groups;(3)The hydroxyproline(HYP) content ofcallu tissue had been obviously improved in C group than other groups.The differencebetween C group and other groups was also prominent (p＜0.05). effective for treating bone coloboma healing in rabbits, and has a certain clinical applicationvalue.
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