Synthesis Of Phosphoryl Dihydroxy-anthraquinones And Research Of Their Biological Activities By Fluorescence

9,10- anthraquinones multi hydroxy derivatives have anticancer function and can inhibit multifarious diseases in organism caused by oxidation reactions. Many medical molecules have many unique activities and characteristics after being phosphorylated. It is very important to synthesis new phosphoryl dihydroxy-anthraquinones and research interaction detween them and biological macromoleculer for exploration of new drug.In this thesis,by Atheron-Todd reaction ,using diethyl phosphate and diisopropyl phosphate as phosphorylate reagents,we successfully synthesized a series of new dihydroxy-anthraquinones phosphate derivatives. The synthesis route was shown as following scheme. Their structures were confirmed by NMR, IR and ESI-MS. It was found that the reaction activities ofβ-hydroxyl multi- anthraquinones is much higher than those ofα-hydroxyl, which have been further confirmed by charge distributing analysis.The interactions of phosphoryly dihydroxy anthraquinone and BSA was confirmed by Fluorescence Analysis, which coubld be further used as a a powerful tool for screening numerous small molecule-protein complexes, due to its rapidity of data acquisition and the relative simplicity of interpretation. The results show that the binding constants between them are all about 10~5, and the number of bind site is 1 respectively, and the type of applied force between them is Van-der Waals Force and hydrogen bond.