| Objective To study the protective effect of quercetin (Que) against paraquat (PQ)-induced Acute lung injury and its mechanism.Methods All mice were randomly divided into three groups and there were twenty mice in each group: normal control group, PQ group, Que group. Lung injury was observed at the times of 8, 24, 48 and 72h respectively. SD rats were given (intraperitoneal, i.p.) Que(100mg/kg) at 3h after intraperitoneal of PQ (50mg/kg). The level of MDA, superoxide dismutase (SOD) and GSH-Px both in plasma and bronchoalveolar lavage fluid(BALF) were detected at 8 ,24 ,48 ,72h respectively after Que treatment. The expression of NF-KBp65 in lungs was measured by immunohistochemistry staining, and wet/dry weight (W/D) were recorded.Results Compared with control group, the level of MDA in PQ group increased significantly both in plasma and BALF (P<0.01); SOD and GSH-Px in plasma were all decreased (P<0.01). The activities of SOD and GSH-Px in BALF were all decreased, but SOD showed increase 48h later. Compare Que group with PQ group ,the MDA level decreased both in plasma and BALF(P<0.05), the SOD and GSH-Px activities were increased as well (P<0.05). The pathological changes in lungs caused by PQ were mainly shown as congestion, leukocyte infiltration, thickness and local hemorrhage in alveolar wall. After Que treatment, congestion and hemorrhage of alveoli got some improvement. Compare Que group with PQ group, the pression of NF-KBp65 in lungs and W/D of Lungs were all decreased (P<0.05).Conclusion 1.There were the oxidant-antioxidant unbalance in PQ rats, oxidative stress may play an essential role in the progress of acute lung injury. 2. Quercetin can effectively inhibits oxidative stress and have protective effects on Paraquat-induced acute lung injury in rats.
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