Shock Wave Promotes Differentiation Of Human Mesenchymal Stem Cells Into Osteoplasts

Objective: To study the infect of shock waves on the differentiation of the human mesenchymal stem cells (hMSCs) and find the best condition of SW to promote hMSCs to differentiate into osteoprogenitor cells and explore the mechanism.Methods: The hMSCs derived from the bone marrow pool collected from healthy human volunteers. The cells were isolated and cultured in vitro by density centrifugal and anchoring screening. The cells were identified by immunohistochemistry and induced by SW of different doses. The hMSCs differentiated into osteoprogenitor cells were evaluated by alkaline phosphatase Ca-Co staining, calcium node formation and Collagen type I immunohistochemisty 40 days later. An optimal dose of SW was selected to treat hMSCs. RT-PCR was used to evaluate the expression of c-fos and c-jun.Results: The best energy dose of SW treating hMSCs was 8.5 kV (120). It was showed that osteogenesis was clear in SW group. The expression of c-fos and c-jun grew more and reached climax after 45 minutes.Conclusion: SW could promote hMSCs to differentiate into osteoprogenitor cells. The expression of c-fos and c-jun grew more.