Visible Research Of HL-60 Cell Apoptosis Induced By MNPs-ASOs Complex

Antisense technique is an important area of research in cancer-associated treatment. Compared with conventional cancer chemotherapy, antisense therapy is less cytotoxic side-effects based on the identification of cancer-associated molecular sites, which could allow the selective targeting of cancer cells without suppressing normal cells. The principle of this technology is the sequence-specific binding of an antisense oligonucleotide (ASOs) to target mRNA, thereby preventing gene translation. ASOs are one of such class of agents for inhibiting gene expression and resulting in cell apoptosis.The application of magnetic nanoparticle-ligand targeting systems for drug delivery is of particular interest. Acting as a vehicle to specifically deliver cancer-fighting drugs to tumors, magnetic nanoparticles show great promise for research towards clinical diagnostic and therapeutic applications. ASOs-SMNPs conjugation is thus a considerably exciting targeting delivery system for specific cellular uptake and selective tumor targeting.An efficient method using magnetic targeting delivering drug system(MNPs-ASOs complex) was founded in this paper. The MNPs-ASOs complex was used to induce the HL-60 cell and the apoptosis was studied by AFM, TEM, etc. The main works were done as follow:1. Quantity-efficiency and time-efficiency and diameter-efficiency experiment were done to assure the safety of the nanoparticles and the optimal concentration and the best time, which would give the preparation of the next research about the apoptosis of the cells inducted by the ASOs-MNPs complex. 2. The magnetic nanoparticles were wrapped by silicon, then the ASOs-MNPs complex was prepared by the reaction of 19-mer-ASOs with thiol-modified SMNPs. The HL-60 cells were induced by the ASOs-MNPs complex including quantity-efficiency and time-efficiency experiments. MTT, TEM, AFM, Fluorescence Microscopy, FCM, etc. were applied to characterize the cell apoptosis including quantity and quality.A new characterization was adopted using atomic force microscope observing the cell apoptosis. TEM and Fluorescence Microscopy are also exploted to observe the apoptosis. These operations made the cell apoptotic morphology visible. The magnetic nanoparticles used as vectors greatly improve the cell transfection, which upgrade the cell apoptotic rate in a large extent. SMNPs–mediated administration system has long been considered to be a high-efficiency target delivery. Antisense oligonucleotides technology is one of the most promising therapeutic strategies to prevent the progress of diseases through inhibiting the specific gene expression.