Effect Of Sonodynamic Therapy On Three Different Plasma Membrane Morphologic Structure And Fluidity Of Ascites Tumor Cell

Malignant tumor is one of the most deadly diseases for human Being. Scientists from both domestic and overseas having been done many researches to conquer it. The normal methods of the tumor treatment mainly includes resection, chemotherapy, radiotherapy, thermotherapy, hormonoe therapy, photodynamic therapy(PDT) and so on. While in the ultrasound antitumor fields, there concerns about high intensity focused ultrasound(HIFU)surgery, ultrasound thermal treatment and sonodynamic therapy(SDT). Sonodynamic Therapy (SDT) is a new anti-tumor therapy brought forward by Japanese scholars Umemura et al in 1989. The antitumor effect of SDT is based on the synergistic effects induced by photosensitizer which can remain preferentially in tumor tissues much longer than in normal tissues when activated with ultrasound. Many scholars have paid close attention to SDT for its theoretical significance and clinicalvalue. Due to the special accumulation of hematoporphyrin derivatives in tumor cells and the selective irradiation of the ultrasound. SDT has no damage on healthy tissues and avoided chemotherapy, radiotherapy side-effect, etc. So is suitable for many patients who can not be cured with the operation or in need of chemotherapy through veins. So the application of sonodynamic treatment is a promising way on treatment of tumors in deep tissue.At the present time, scholars have been done extensively and deeply investigating of this therapy, including the mechanism such as cavatition, singlet oxygen, hydroxide radicals, alkyloxygen radicals, choice of sonosensitizer such as antitumor drug, porphyrin, and other compounds, morphologic changes after SDT treatment showing there are two effect models: necrosis and apoptosis, and other histochemistry studies. But currently using equipment with nanometer resolution to observe the change of morphologic structure of cells induced by sonodynamic therapy on tumor cell apoptosis and other physics attribute of cells research rarely. In this dissertation, 1.43MHz, 5W/cm~2 is used to study the effect of sonodynamic therapy on plasma membrane ultrastructure of ascites S180 tumor cell, ascites EAT tumor cell, ascites H-22 tumor cell, on the background of international research development and our previous results and this research is supported by national natural science foundation of China. Presently, the results got as follow:1, Effect on morphologic structure of ascites tumor cells: using the atomic force microscopy(AFM) to observe effect of sonodynamic therapy on membrane morphologic structure of ascites S180 tumor cell, ascites EAT tumor cell, ascites H-22 tumor cell. After SDT treated, the damages degree of three kinds of ascites tumors cells are diffent, but actually, the change of morphological characteristic displays the certain regularity: CT group approaches spherical cells, the membrane is smooth and integrity; U group is similar to CT group, no significant changes in the membrane surface. Several membrane rupture. Parts of ceils are damaged in U group alone, the irregular shape of the cells were damaged and there are several vesicles on the membrane, membrane incomplete, and that there are depression; Serious damage to the cells of UH groups. Some cells sharp are extremely irregular. Most of the cell membrane damaged, damaged cell structure disintegrated which have severl apoptotic bodies. Most of the cells showed significant apoptosis features, with the time extension, the phenomenon of apoptosis is more evident. Injury results in the four groups is the most obvious;2, Effect on fluidity of ascites tumor cell: Use time-correlated single photon counting fluorescence spectrometer to observe effect of sonodynamic therapy on membrane fluidity of ascites S180 tumor cell, ascites EAT tumor cell, ascites H-22 tumor cell. According to the results, SDT can evidently to drop the three tape of membrane fluidity. The change of the membrane fluidity inevitably affects a series normal functions of cells, thus is possible to be indirect or the direct restrain the malignant multiplication of tumor cells.