Detection Of The Correlation Between Gene Mutation Of TSG101 And Gastrointestinal Cancer In Peripheral Blood Leucocytes Of People

Objective: To investigate the expression of gene mutation in exon 4 of tumor susceptibility gene(TSG101) in peripheral blood leucocytes of healthy adults and gastrointestinal cancer patients in order to explore the clinical significance of the gene mutation in exon 4 of gene TSG101.Methods: Genomic DNA was isolated from peripheral blood cells donated by 78 healthy adults and 40 gastrointestinal cancer patients. Using specific primers for exon 4 of TSG101, PCR amplification of genomic DNA was performed. Agarose electrophoresis was performed to identify the PCR products. Each products was sequenced with primers and compared with the sequence of gene TSG101 issued in genebank and performed correlated analysis with the clinical data.Result: 1.The expression of mutation rate in exon 4 of TSG101 was detected in 18.92% of 37 health women, 26.83% of 41 health men, respectively. Among the total, sequence results showed that a same "C→T" heterozygous point mutation, at 798bp of exon 4, was detected in 7 samples in women and 10 samples in men; only 1 homozygous point mutation was detected in male at the same position. There presented no distinct difference between health women and men of mutation rate in exon 4 of TSG101(P＞0.05). 2. The expression of mutation rate in exon 4 of TSG101 was detected in 34.25% of 40 gastrointestinal cancer patients. Among the total, 9 heterozygous point mutation and 5 homozygous point mutation was detected at the same position. There presented no distinct difference between healthy adults and gastrointestinal cancer patients of mutation rate in exon 4 of TSG101(P＞0.05), but homozygous point mutation rate in gastrointestinal cancer patients is obviously more than in healthy adults(P＜0.05).Conclusions: 1. Heterozygous and homozygous gene mutation in exon 4 of tumor susceptibility gene(TSG101) were detected in peripheral blood cells of healthy adults and gastrointestinal cancer patients. 2. Homozygous point mutation rate in gastrointestinal cancer patients is obviously more than in healthy adults at 798bp of gene TSG101, it was firstly reported worldwidely. 3. That gene mutation in exon 4 of TSG101 were detected in peripheral blood leucocytes of healthy adults and gastrointestinal cancer patients hints a SNP may exist in exon 4 of gene TSG101. Detecting gene mutation of exon 4 of gene TSG101 might imply a new clue to the molecular genetic mechanism of oncogenesis of gastrointestinal cancer.