Preparation And Characterization Of The Injectable N-methylene Phosphonic Chitosan/Alginate Hydrogel

To overcome the obstacle of mechanical property and artifactitious performance of thenatural hydrogel, this dissertation studied firstly N-methylene phosphonic chitosan(NMPC) forhydrogel material. The experiment was designed to prepare better injectable hydrogel underensuring biocompatibility of polysaccharides. The accurate concentration of differentcomponents is discovered by the way of orthogonal design experiment for mechanical property,combining with the analysis of pH value, hydrogel-forming time and swelling coefficient. Thebest point for the natural hydrogel is found out. Finally, the cell culture in vitro and the plantationin vivo experiment were conducted to evaluate the biocompatibility of the natural hydrogel.In this study, N-methylene phosphonic chitosan was synthesized via the homogeneousphase reaction in a step. Through the test of FFIR, ¹H-NMR, ICP and molecular weight analysis,the result shows that the methylene phosphate radical (-CH₂-PO₃H₂) has successfully graftedonto the amino of chitosan.The natural hydrogel were prepared by injection moulding, by volume ratio of NMPC-CA:ALG-CaCO₃ equal to 1:2. The experiment has discovered (w/v) 3%NMPC, 0.6%CA, 2%ALG,0.5% CaCO₃ are the accurate components percentage in the two different solutions by the way oforthogonal design.At last the study of the cell culture and the plantation in vivo experiment turn out that theNMPC and the natural hydrogel have made cell proliferation apparently, found no the fibrousenvelope and the inflammatory reaction in vivo. The natural hydrogel with goodcellcompatibility and tussiuecompatibility confirmed, can be applied in repairing the defect ofbone and the carrier of drug delivery etc.