Screening Of Mycobacterium Tuberculosis Pathopoiesis Genes In Xinjiang Clinical Strains

Objective: Screen differential Mycobacterium Tuberculosis genes between Xinjiang clinical strains and H37Rv by suppresssion subtractive hybridization and analysis the function of these specifically pathopoiesis genes .Methods: Both Mycobacterium Tuberculosis Xinjiang clinical strains and H37Rv as tester and driver each other,most identical genome was drived whereas some distinctive genes was remained and enriched by utilization SSH technique.Meanwhile through inversion differential genes to E. coli to determine all of sequences that we have cloned by Blast in Genebank. Analysis the function of differential genes between Mycobacterium Tuberculosis H37Rv and Xinjiang clinical strains.Results: We have cloned all of 16 different sequences and analyzed them.Six different DNA fragments only in Xinjiang Clinical Strains were obtained.One is the fragment of a gene coding monooxygenase, flavin-binding family identified by Glimmer2;one fragment belongs to acyltransferase family protein;one for aminotransferase, class II,acyl carrier protein; one fragment belongs to chromosomal replication initiator protein DnaA and one for M.tuberculosis paralogous family11-pyridoxamine 5'-phosphate oxidase-related; Meanwhile we have cloned ten DNA fragments only in Mycobacterium Tuberculosis H37Rv which we have known .Conclusions: SSH technique can efficiently screen differential genes in M. Tuberculosis in Xinjiang Clinical Strains.They are possible key genes that Mycobacterium Tuberculosis survive and fortify virulence in mal-environment.