Study For Mutations Of A Campylobacter Jejuni Strain Associated Guillain-Barré Syndrome

Campylobacter jejuni (C.jejuni) is the most frequent cause of bacterial diarrhea. Guillain-Barrésyndrome (GBS) is a severe complication after C. jejuni infect. Recent neurophysiological and pathological studies have led to a reclassification of GBS into acute inflammatory demyelinating polyneuropathy(AIDP),acute motor axonal neuropathy(AMAN),acute motor and sensory axonal neuropathy(AMSAN),Miller-Fisher syndrome (MFS). The development of GBS are associated with certain C.jejuni strains with specific serotype.The common strain is Penner O:19 in our country.In our study, we analyze mutation of GBS-associated C.jejuni which isolated from a GBS patient in our northern area of China. We search the different between NCTC11168 and GBS-associated C.jejuni at flaA and wla cluster. The aim of this study was to investigate the relation of Guillain-BarréSyndrome and Campylobacter jejuni mutations.MethodsBacteria culture:The Cj strain we used was isolated from AMAN patient,and identified with AMAN-associated strain by model animals,such as chicken and monkey.The serotype is Penner O:19. Culture condition were following the procedure of Li CY et al.DNA isolation: DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).PCR: PCR reactions were performed as described previously.All PCR products were analyzed by agarose gel electrophoresis.Sequence: All PCR products sequenced by Shanghai Sangon Biological Engineering Technology Corporation.Sequence analysis:DNA sequence identity with C. jejuni NCTC 11168 were identified by BLAST of NCBI database, and DNA star 4.0 Software.Result1 In our study,campare with NCTC 11168 sequence, which displays more than 97% identity.2 The number of nucleotide mutations in fla gene and wla cluster is variable. The most nucleotide mutations is in flaA gene and wlaF, separate 15 nucleotide mutations and 20 nucleotide mutations.3 The amino acid mutations is similar nucleotide mutations. The most amino acid mutations is in flaA gene and wlaF, separate 13 amino acid mutations and 60 amino acid mutations.Besides wlaF absent 5 nucleotide,so result in a series of amino acid mutationsConclusionIn our study,there were a number of nucleotide and amino acid mutations in fla gene and wla cluster by identity with NCTC 11168 sequence. The finding will help us to understand the molecular pathogensis of GBS-associated C.jejuni,and established fundament for explored the biologically characteristic of GBS-associated C.jejuni.