| Objective: Using with glucocorticoid and immunodepressive drug in these years, the life of Rheumatoid Arthritis(RA) patients has been prolonged. At the same time, the number of RA patients with cardiovascular disease is increasing. And cardiovascular disease has became the first reason in RA patients'death. Thus, investigating the mechanism in it and the precautionary measure became one of the cardinal problem in RA clinical treatment. The aim of the study is to investigate the effect of QingXinYin to relaxant response depend on endothelium of isolated aortas, homocysteine(Hcy), vascular cell adhesion molecule-1(VCAM-1), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and endothelin-1 (ET-1) to investigate the protective mechanism and afford experimental evidence for clinical work.Methods: Choose Wistar rats weight from 150g to 180g, the number of male and female is equal. Injecting Freund's complete adjuvant(FCA) into the subcutis of their right rear paws after sterilizing regularly is to found AA rat models and ten of rats are injected equivalent volume of saline. On the 19th day after injecting, rats that presenting secondary response are divided randomly into Model group, MTX group, MTX&QXY group, and each group includes 10 samples. The rats injected saline were as control group. After measuring the paw volumes and weights of rats in each group, the treatment rats are given drug for successive 21 days. On the second day after treatment is over, after measuring the paw volumes and the weights, rats are sacrificed by withdraw of blood by abdominal aortas under 3% pentobarbital sodium anesthesia to get serum and plasma. ET-1, TNF-α, IL-1βare determined by radioimmunoassay, VCAM-1 by ELISA, Hcy by cyclical zyme technique. After drawing blood, thorax was cut open and removed thoracic aortas quickly. Then, it was cleared of adherent fatty and connective tissue, and was scissored rings of 3-4mm length. Determining relaxant response depend on endothelium by technique of aortas in organ chambers. The remanent part of aortas was fixed, dehydrated, wrapped and dyed to determine VCAM-1 in vascular endothelium cell by immunohistochemical.Data Statistics: All data is analysed by SPSS for windows 13.0 and the results are expressed as X±S. The comparison of mean among groups is evaluated by one-way analysis of variance(ANOVA)Results:1 The effect of MTX&QXY to general condition of AA rats Comparing with model group, MTX group and MTX&QXY group are increased in general condition. After model founded, the weight of model group, MTX group and MTX&QXY group is lower than control group significantly(P<0.01), but there is no significant difference among the AA three groups. At the end of the experiment, the weight of model group, MTX group and MTX&QXY group is still lower than control group significantly(P<0.01), model group and MTX group is lower than MTX&QXY group significantly(P<0.01), and model group is lower than MTX group significantly(P<0.01). The augment of MTX group and model group is lower than control group and MTX&QXY group in weight significantly(P<0.01), model group is lower than MTX group significantly(P < 0.01), but there is no significant difference between control group and MTX&QXY group(P>0.05).2 The depressive effect of QXY to paw swelling of AA rats At the end of experiment, the average swelling degree(%) of FCA-injected paws of MTX group and MTX&QXY group is lower than model group significantly(P<0.01), and MTX group is parallel with MTX&QXY group(P>0.05). The average swelling degrees(%) of non-FCA-injected paws of MTX group and MTX&QXY group is lower than model group significantly(P < 0.01), and MTX group is parallel with MTX&QXY group(P>0.05).3 The effect of QXY to relaxation depend on endothelium of AA ratsThe acetylcholine(Ach) induced endothelium dependent relaxation are decreased in aortas from AA rats, and model group and MTX group is lower than MTX&QXY group significantly(P<0.01), model group is lower than MTX group significantly(P<0.01 or 0.05). The sodiumnitroprusside(NaNP) induced endothelium independent relaxation has no significant difference among groups in AA rats(P>0.05).4 The effect of QXY to TNF-αand IL-1βin serum, ET-1 in plasma and VCAM-1 in serum and vascular endothelium cell of AA rats4.1 Comparing the level of TNF-αand IL-1βin serum, model group and MTX group is higher than control group significantly(P<0.01), model group and MTX group is higher than MTX&QXY group significantly(P<0.01), model group is higher than MTX group significantly(P<0.01), but there is no significant difference between MTX&QXY group and control group(P>0.05).4.2 Comparing the level of ET-1 in plasma, model group ,MTX group and MTX&QXY group is higher than control group significantly(P<0.01), model group and MTX group is higher than MTX&QXY group significantly(P<0.05), and there is no significant difference between model group and MTX group(P>0.05).4.3.1 Comparing the level of VCAM-1 in serum, model group and MTX group is higher than control group significantly(P<0.01), model group and MTX group is higher than MTX&QXY group significantly(P<0.01), model group is higher than MTX group significantly(P < 0.01), and there is no significant difference between MTX&QXY group and control group(P>0.05).4.3.2 Comparing the level of VCAM-1 in vascular endothelium cell, model group and MTX group is higher than control group significantly(P<0.01), model group and MTX group is higher than MTX&QXY group significantly(P<0.05), model group is higher than MTX group significantly(P<0.01), and there is no significant difference between MTX&QXY group and control group(P>0.05).4.4 Comparing the level of Hcy in serum, model group, MTX group and MTX&QXY group is higher than control group significantly(P<0.01), model group and MTX group is higher than MTX&QXY group significantly(P<0.01), and there is no significant difference between model group and MTX group(P>0.05).Conclusions:1 Hcy and VCAM-1 in serum and ET-1 in plasma are all increased significantly, and relaxation dependent on endothelium is decreased significantly. They all confirm that AA rats have many factors engendering cardiovascular dysfunction.2 QingXinYin can resist inflammation positively. QingXinYin combine with MTX can remedy AA significantly, decrease the paw swelling and the level of inflammatory cell factors such as TNF-αand IL-1β.3 QingXinYin can protect vascular dysfunction of AA rats. QingXinYin increase relaxation dependent on endothelium, decrease ET-1 in plasma, VCAM-1 and Hcy in serum and VCAM-1 in vascular endothelium cell to protect endothelium dysfunction in AA rats'vascular.
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