The Effects Of Centipede Acidic Protein On Anti-atherosclerosis And The Influences Of The Atria And Thoracic Aortic Rings

Objective: To investigate the effects of Centipede Acidic Protein (CAP) on anti-atherosclerosis in rats, the spontaneous beating, the contractility of atria and the thoracic aortic rings in guinea-pigs.Methods:Part 1 The experiment study of CAP on anti-atherosclerosis1 The extraction of CAPFirstly, Centipedes were grinded powder, 95% alcohol was added, then CAP was obtained by a series of steps (such as centrifuge, filtration, etc.). At last, the pH of CAP was adjusted to be 4.4 by phosphoric acid (concentration: 1mol/l). The concentration of crude drug of Centipede was 0.484 g/ml, which of the protein was 15.8 mg/ml. It was saved in a freezer at 4℃.2 The experiment of CAP on anti-atherosclerosis60 Sprague-dawley rats were divided randomly into 5 groups: normal group, model group, simvastatin group, low dose CAP group (L-CAP) and high dose CAP group (H-CAP). Simvastatin group treated with simvastatin, L-CAP group treated with low concentration CAP (0.242 g/ml), H-CAP group treated with high concentration CAP (0.484 g/ml). Saline was given to rats of normal group and model group at the same volume in the same way. The normal group rats were fed with standard chow; other groups were administrated with vitamin D3 for three days, then fed with high fat diet for 30 days. The hemodynamics, hemorheology, blood-fat, sero-enzyme were examined in the end of the experiment. Morphological changes of aorta and liver were observed under gross and light microscopes.Part 2 The effects of CAP on the spontaneous beating and the contractility of atriaThe atria were prepared from 30 guinea-pigs (200~240g) and the effects of different concentrations of CAP on the spontaneous beating, the contractility were observed in the absence and presence of different blocking agents.Part 3 The effects of CAP on the tension of thoracic aortic rings in guinea-pigsThe thoracic aortic rings were prepared from 30 guinea-pigs (200~240g) and the effects of different concentrations of CAP on the contractility of thoracic aortic rings were observed in the absence and presence of different blocking agents.Results:1. Compared with normal group, the lv+dp/dtmax was lower and t-dp/dtmax was longer in the model group. The lv+dp/dtmax of simvastatin group, L-CAP and H-CAP group was higher, t-dp/dtmax was shorter compared with model group. 2. The levels of TC, TG, LDL in plasma were higher and that of HDL was lower in the model group compared with the normal group. In simvastatin group, L-CAP and H-CAP groups, the levels of plasma TC, TG, LDL decreased and HDL increased compared with model group.3. The LSFB, HSFB, ESR, PV and HCV were higher and that of DFI was lower in the model group compared with the normal group. In simvastatin group, L-CAP and H-CAP group, LSFB, HSFB, ESR, PV, HCV decreased and DFI increased compared with model group.4. The ET, MDA increased in plasma markedly and SOD, NO decreased obviously in the model group compared with normal group; In simvastatin group, L-CAP and H-CAP group, the ET, MDA decreased markedly and SOD, NO increased obviously compared with model group.5. The obvious changes of atherosclerosis were shown in the model group: there were focal or transient areas of endothelium loss, monocyte adhered to the sub-endothelial space or moved into the vascular wall, et al. Those changes of aorta endothelium in simvastatin group, L-CAP and H-CAP groups were less compared with model group.6. The effects of CAP on the spontaneous beating, the contractility of atria and the tension of thoracic aorta of guinea-pigs.CAP produced the positive inotropic effect and chronotropic effect in concentration-dependent mannars in atria from guinea-pigs. Thses effects could be blocked partially byβadrenoceptor antagonist propranolol (Pro), orαadrenoceptor antagonist phenotolamine (Phe).CAP markedly increased the tension of thoracic aorta of guinea-pigs in concentration-dependent mannars. Thses effects could be blocked partially byαadrenoceptor antagonist Phe and calcium antagonist nifedipine (Nif).Conclusion:CAP can prevent from the progression of atherosclerosis by interfereing with many links of atherosclerosis, and increase the spontaneous beating, enhance the contractions of myocardium and thoracic aorta. This study may set a theoretical foundation for its application in clinical cardiovascular diseases.