Neuroprotective Effects Of EPO On Primary Cultured Rat's Neurons Following Ischemic-like Conditioning

Objective:To investigate the damage of ischemic-like on the primary cultured rat's neurons,the protective effects of EPO and the optimal dosage.Methods:Cortical neurons cultured for 7 days were randomly divided into five groups:control group,ischemic-like group and plus three kinds of concentration of EPO groups treated.Then morphology of neurons was observed under inverted microscope.The survival rates of neurons at different groups were measured with MTT reduction assay and LDH examination.The apoptosis of neurons was detected using flow cytometry.Statistical analysis were using SPSS 12.0 software.Statistical analysis fixed that P＜0.05 is significant.Results:We established a experimented cultured rat's cortical neurons by using the commonculture method.The cortical neurons was detected by neuronal specific enolase(NSE) immunocyto-chemical staining.We established a model by the mothed of ischemic-like conditioning on the cortial neurons.There was a notable difference between the ischemic-like group and the groups plused EPO(P＜0.05).The medium concentration EPO could reduce apoptosis of neuron and increase survival rates of neuron.But the lower and the high concentration EPO groups exert less action although having neuroprotective effects also.Conclusion:Through the flow cytometry,MTT reduction assay and LDH examination,we find that using the medium concentration EPO can reduce the damage of neurons caused by ischemic -like through reducing neuron apoptosis and increase the survival rate of neurons.What suggests that EPO has an effective neuroprotection.