The Changes Of NF-κB And TNF-α In Diabetes Mellitus Rat's Kidney Following Limbs Ischemic Reperfusion And The Intervention Effects Of Propofol

Objective To investigate the mechanism of action of cytokine and nuclear factor in normal rats and diabetes mellitus rats following limbs ischemic reperfusion and the mechanism of protect action of Propofol,the experiment was made on the changes of nuclear factor of kappa B(NF-κB)and tumor necrosis factor-α(TNF-α)in diabetes mellitus rat's kidney following limbs ischemic reperfusion and the effect of Propofol in the process.Method Adult male Ssprague-Dawley rats,weighing 220-250g were randomized assigned to normal rats and diabetes mellitus rats.The rats were induced diabetes mellitus with streptozotocin(STZ).The normal rats were divided into three groups at random:groupⅠA(n=8) control group,groupⅠB(n=8)limbs ischemic reperfusion group,groupⅠC(n=8)limbs ischemic reperfusion and Propofol group.Diabetes mellitus rats were divided into four group at random:groupⅡA(n=8)control group,groupⅡB(n=8)limbs ischemic reperfusion group, groupⅡC(n=8)Propofol group,groupⅡD(n=8)limbs ischemic reperfusion and Propofol group.the exprement deployed immunohistochemistry(IHC)in order to detect qualitatively and semiquantitatively the express of TNF-αand NF-κB.and enzyme-linked immunosorbentassay (ELISA)in order to detect quantitative the level of TNF-α.Result First,limbs ischemic reperfusion(groupⅠB)can cause renal damage.compared with normal rat(groupⅠA),the level of NF-κB and TNF-αis increased and there is statistical significance(P＜0.05).Second,diabetes mellitus rats(groupⅡA)can cause renal damage.compared with normal rat(groupⅠA),the level of NF-κB and TNF-αis increased and there is statistical significance(P＜0.05).Third,diabetes mellitus with limbs ischemic reperfusion can cause renal significant damage,compared with normal rat(groupⅠA)and limbs ischemic reperfusion(groupⅠB)and diabetes mellitus rats(groupⅡA),the level of NF-κB and TNF-αis predominantly heighten,and there is statistical significance(P＜0.05).Fourth, compared with the AP:PA of intervention of Propofol,every group level of NF-κB and TNF-αis cut down after intervention of Propofol and there are statistical significance(P＜0.05).Conclusion First,the level of NF-κB and TNF-αwas step up for diabetes mellitus rat,limbs ischemic reperfusion rat with diabetes mellitus and limbs ischemic reperfusion rat without diabetes mellitus.Moreover,the level is maxlmum for diabetes mellitus with limbs ischemic reperfusion rat.Second,Propofol can cut down the level of NF-κB and TNF-αfor diabetes mellitus rat and limbs ischemic reperfusion rat's kidney.it is to say that Propofol with anti-inflammatory and antioxidative role has protection of the renal damage induced by limbs ischemic reperfusion injury and diabetes mellitus injury.