Study On The Relationship Between Microsatellite Alterations Of FHIT Gene And HPV₁₆ Infection And Cervical Carcinoma

Objective:To explore the relationship of microsatellite alterations of FHIT gene to the development of cervical carcinoma,and its relationship with HPV₁₆infection.Methods:Two sites of microsatellite polymorphism of FHIT gene were selected,we use polymerase chain reaction(PCR)technique to detect LOH and MI in 50 cases of cervical carcinoma and 40 cases of cervical intraepithelial neoplasia(CIN),and to detect the infection state of HPV₁₆.Results:At D3s1234,the LOH rates of cervical carcinomas were 46%(23/50),the LOH rates of CINs were 40%(16/40).There were no significant differences between cervical carcinomas and CINs(P＞0.05).The MI rates of cervical carcinomas were 18%(9/50),the MI rates of CINs were 12.5%(5/40)(P＞0.05).There were no significant differences between cervical carcinomas and CINs.At D3s4103,the LOH rates of cervical carcinomas were 40%(20/50),and the LOH rates of CINs were 37.5%(15/40)(P＞0.05).There were no significant differences between cervical carcinomas and CINs.The MI rates of cervical carcinomas were 14% (7/50),and the MI rates of CINs were 7.5%(3/40)(P＞0.05).There were no significant differences between cervical carcinomas and CINs.In 50 cervical carcinomas,the positive rates of LOH for HPV₁₆infenction were 79.5%(35/44),and MI were 31.8%(14/44);the negative rates of LOH were 33.3%(2/6),MI were 16.7%(1/6).There were significant differences between HPV(+)and HPV(-)in respect to their positive rates of LOH(P＜0.05),and no significant differences of MI(P＞0.05).In 40 CINs,the positive rates of LOH for HPV₁₆ infenction were 82.4%(14/17),and MI were 35.3%(6/17);the negative rates of LOH were 52.2%(12/23),MI were 17.4%(4/23).There were significant differences between HPV(+) and HPV(-)in respect to their positive rates of LOH(P＜0.05),and no significant differences of MI(P＞0.05).Conclusion:There were relationship between microsatllite alterations of FHIT and the development of cervical carcinoma.The FHIT gene change is a relatively early event in the development of cervical carcinoma.LOH of FHIT correlated with the infection of HPV₁₆.