Study On The Expression And Methylation Status Of IL-13Rα1 Gene In SLE T Cells

Objective:To investigate the IL-13Ra1 mRNA expression and its epigeneticregulatory mechanisms in SLE T cellsMethods:(1) PBMC (peripheral blood mononuclear cells) cells were isolatedfrom the peripheral venous blood of SLE patients and healthy donors bydensity gradient centrifugation. CD4+ and CD8+ T cells were isolatedusing Miltenyi beads and protocols provided by the manufacturer.(2) Genomic DNA, mRNA were isolated.(3) The expression of IL-13Ra1 mRNA was investigated withquantitative real time PCR.(4) Purified DNA was treated with sodium bisulfite, then detect themethylation status of IL-13Ra1 using MSP(methylation specific PCR)Results:(1) The IL-13Ra1 mRNA expression in SLE CD4+ and CD8+ Tsubsets were significantly higher than that in normal subsets (P＜0.05,P＜0.05)(2) Compared with normal cells, IL-13Ra1 in SLE CD4+ T cells ishypomethylated. (P＜0.05)(3) In SLE CD4+, CD8+ T cells, the IL-13Ra1 mRNA expressionis positively correlated with SLEDAI score, and the methylation levelof IL-13Ra1 is negatively correlated with SLEDAI score. In SLE CD4+ T cells,the IL-13Ra1 mRNA expression is negatively correlated with itsmethylation level.Conclusion:Overexpression of IL-13Ra1 caused by hypomethylation in T cellsmight be involved in the pathogenesis of SLE.