| Objective To study the clinical value of the phage amplifiedbiologically assay (PhaB) on rapid detection of Mycobacterium tuberculosisin pus of pott's disease.Methods 56 cases selected in this experiment were clinicaldiagnosed by history, symptoms and imaging signs during the periodbetween August of 2006 to March of 2007. All the specimens werecollected during the operation on the principal of asepsis. 10 clinicalisolates of Mycobacterium tuberculosis, 15 strains of non-Mycobacteriumtuberculosis, 5 strains of non-mycobacterium and 56 pus specimens ofpott's disease patients were detected with AFB test, rapid culture withBACTEC MGIT-960 system and phage amplified biologically assay.Results All the strains of Mycobacterium tuberculosis clinicalisolates detected with phage amplified biologically assay were positive,while all of the non-Mycobacterium tuberculosis and non-mycobacteriumstrains were negative. 46 pus specimens with BACTEC MGIT-960culture positive and 10 pus specimens with BACTEC MGIT-960negative were detected with phage amplified biologically assay, thenumber of positive specimens was 42(91.3%) and 3 (30.0%) respectively,The rate of positive were 82.1% and 80.4% respectively(P＞0.05). Thepositive number of smear was only 13 (23.2%), negative was 43(76.8%). However, the number of positive specimens detected by phage amplifiedbiologically assay was 45(80.4%), 30 of them were negative detected bypus smear (P＜0.05). The result of PhaB could be reported in 18-24hours, 5-17 days were advanced compared with BACTEC MGIT-960.Conclusions The phage amplified biologically assay can detectthe Mycobacterium tuberculosis more easily and quickly with highsensitivity and specificity compared with direct smear and BACTECMGIT-960. The whole process just needs one day. It appears a wellprospect in diagnosis and treatment of Pott's disease. |
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