The Precess Research Of Labelling Peptide And Antibody With Y

This experiment is aimed at searching for the best experimental conditionfor labelling monoclonal antibody with the radionuclide Y and the method oflabelling, for application of the cell and animal experiment later, and the resultwill be used in clinical trial finally.The reseach of this experiment is a goodreference for clinical trial study.This experiment adopt indirect method for labelling Y to peptide andantibody, we use as DOTA and p—SCN—Bz—DOTA as Bifunctionalchelating agents.We synthesize DOTA in the experiment,and use new method- microwavetechnology to remove tosyl group.Campared with the general heatingmethod,this method can save reaction time to 1/4000 of the generalmethod,increase the efficiency of the reaction.Coupling octrotide with bifunctional chelating agents-DOTA, but theoutgrowths of this reaction are so many and the purification step is so complexthat we can not achieve satisfied result of coupling and labelling. We use different methd, such as IR, NMR and so on to identify theproduct.We use p—SCN—Bz—DOTA to replace DOTA as theBifunctionalchelating agents-DOTA.The effect of labelling octreotide and monoclonalantibody is good. The lablled rate of octratide is 68.3％while the lablled rateof monoclonal antibody is 68.4％.And then detect the lablled rate of antibodyafter 48h, which is 60.4％.The result shows that the labelled antibody is stablein vitro.