The Effect And Mechanisms Of β-C And VE Administration On The Cellular Activities In Vivo And Vitro

Objective: To evaluate the effect and the mechanism of Vitamin E(VE) andβ-carotene (β-C)administration on peripheral blood cells in vivo and on the growth in theEndothelial Cell of Umbilical Vein(ECUV) and the Bone Marrow Stem Cell(BMSC) invitro.Method: 1. A randomized intervention trial ofβ-C was designed. 188 healthy youngpeople aged 19 to 23 years, including 95 males and 93 females, were randomized into 4groups. The controll group was treated with VE 5mg/d as a placebo, other groups wereassigned withβ-C 16.7mg/d,8.35mg/d and 5.57mg/d respectivly for 8 weeks, inaddition to VE 5mg/d treatment. The proliferation of peripheral-blood lymphocyte (PPL)and the hemolytic degree of RBC were detected at the beginning and the end of the trial.2. The cell-study of VE andβ-C in vitro. The ECUV and the BMSC of 4th-6thtransfer of culture in vitro, were divided into 8 groups respectively, i.e.A, B, C, D, E, F, Gand H groups. Each group contained 6 parallel samples. In the study of VE, the group Awas a blank control, the group B was treated with 3.2% DMSO as a solvent control; thegroups C～H were treated with 10μmol/L, 20μmol/L, 40μmol/L, 80μmol/L, 160μmol/Land 320μmol/L of VE respectively. In the study ofβ-C, the blank control was for groupA, and the solvent control for group B was treated with 0.017% CHCl₃+0.32% C₂H₅OH.Other groups were treated with 1μmol/L, 2μmol/L, 4μmol/L, 8μmol/L, 16μmol/L and32μmol/L ofβ-C respectively. After 3 days' treatment, the cell growth curve, activity ofcell proliferation, expression of caspase 3, concentrations of Ca²⁺ and O₂^- in the culturedcells were measured at the end of the study.Result: 1. After the intervention trial, the proliferations of lymphocytes in the groupsupplemented with 16.7mg/d increased 30.4% compared to that before the trail, andincreased 25% compared to the control group (P＜0.05). The hemolytic degree of RBCsignificantly decreased in the group treated with 8.35mg/d ofβ-C (P＜0.05), but there wasnot any significant change of the degree in the 16.7mg/d group. 2. The cell growth curves of the ECUV and the BMSC showed that the cell growthwas decreased in the group H treated with 320μmol/L of VE, and increased in the 2group Ds of 20μmol/L VE treatment. The concentrations of Ca²⁺ and the expressions ofcaspase 3 in the ECUV and the BMSC were both'obviously increased in the 2 group Hstreated with VE compared to the control groups respectively(P＜0.05), and weresignificantly lower in groups D and H than in the solvent controls respectively(P＜0.05).The concentrations of O₂^- in both cultured cells were decreased efficiently in groups Dand C treated with VE compared to the solvent control group respectively (P＜0.05). Butin the 2 group Hs, the concentrations of O₂^- stepped up sharply (P＜0.05).3. After treated with B-C, the cell growth curves of the ECUV and the BMSC weredepressed efficiently in the 2 group Hs (P＜0.05), but were facilitated in the 2 group Ds(P＜0.05). The activities of cell proliferation showed a promotion in the 2 group Dstreated with 2μmol/L ofβ-C on both the ECUV and the BMSC (P＜0.05); Theconcentrations of Ca²⁺ and the expressions of caspase 3 were obviously increased in Thegroups H compared to the control group respectively afterβ-C administration on bothcultured cells respectively (P＜0.05); The concentrations of O₂^- in groups D and C weredecreased efficiently compared to the solvent controls respectively(P＜0.05);Conclusion: The moderate supplementation ofβ-C can improve the proliferation oflymphocytes and decrease RBC hemolyses against H₂O₂ oxidation in young people.Below the dosage of 80μmol/L, the VE could promote the growth and healthy of theECUV and the BMSC cultured in vitro, but it would possibly emerge, adverse effectwhen the concentration comes between 160μmol/L to 320μmol/L. Similarly, whengivenβ-C below 16μmol/L, it would produce a positive effect on the two kinds of cellsin vitro, but induce the apoptosis of cells when it approach to 32μmol/L.