| As an appendix organ of skin,hair has important physiological and sociological functions.The loss of hair pigment and hair itself abnormity, and changes such as density or dexture and extensive hair loss can be sources of influncing appearance and distress to affect individuals.Many elements are involved in the regulation of hair follicle development cycle which control hair growth.The condition in viro made of many factors was unfit for study of cultured hair follicle growth.We had established a model of culturing hair follicle in vitro.By study on hair follicle cell biology,We investigate the mechanism of hair follicle development regulation and factors inflencing follicle growth. The results are not only the theory and experiment bases of hair diseases and wound healing after damag,but also the foundation of tissue engineering development of hair follicle.Focusing on above points some works had been done as follows:①To review the progress of medicine influencing growth of hair follicles.②To establish a relatively ideal model of culturing human hair follicle in vitro.③To observe characteristic of tissue structure in different follicle growth stages on culturing human hair follicle in vitro.④To study the effects of different cultured conditions,minoxidil,spironolactone,insulin-like growth factor-1 on the growth of human hair follicle in vitro by measurement of changes in length of hair follicle with an eyepiece micrometer and by evaluating proliferation of hair follicle cells.⑤To observe cytoarchitecture and morphological changes of hair follicle in cultured follicle growth by pathological analysis.The results as follows:①It was showed that Williams E medium was an excellent medium for hair follicles cultur.The original medium supplements which were insulin,L-glutamine and hydrocortisone were essential for the maintenance of hair follicle growth in vitro.②Hair follicle morphological changes: Before culturing the dermal papilla of hair follicle was clear and the boundarys in dermal papilla,the matrix,the inner root sheath ,the outer root sheath as well as the hair shafts were all clear.The dermal papilla embeded the matrix. In initial stages dermal papilla was still clearly.The elongation of the inner root sheath,the outer root sheath and hair shaft revealed the hair follicle growth.However connective tissue sheath had no changes.Later the boundary between innner root sheath with hair shaft became thicken;In anaphase dermal papilla kept away from matrix and the hiberarchies in hair follicle became more thicken.Hair bulb became contorted and distorted.At last,hair follicle growth stopped and the hair follicle root looked like pestle.③The 0.5mmol/L minoxidil could promote the growth of human hair follicle significantly in vitro.But spironolactone could not either increase or inhibit the growth of hair follicle in vitro significantly;The IGF-1 in concentration of 10ng/ml and100 ng/ml could keep the morphobility of cultured human hair follicle in vitro.The function corresponded with the 10μg/ml insulin.④The pathological slices of hair follicles with HE staining:The dermal papilla of hair follicle which is not cultured had round shape,compact structure, distinct boundary of different tissues.The cultured dermal papilla of hair follicle looked slightness,ascending root,the distance between the fundus of hair root and fundus of hair follicle became larger.The bulk of dermal papilla diminished,loosen structure, more outer root sheath cells.The hair follicle in catagen has illegibility boundaries,the dermal papilla becomes conglobation, the illegibility dermal papilla keeps far from the hair matrix.cells decrease largely,the connective tissue sheath of hair follicle has distinct incrassation. |
