Study On The Biocompatibility Of Novel Poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate)

Terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoat)(PHBVHHx) containing 5.3 tool% 3-hydroxyvalerate (3-HV) and 10.2 tool%3-hydroxyhexanoate (3-HHx) was obtained via microbial synthesis using recombinantAeromonas hydrophila. For the first time in vitro biocompatibility of the terpolyesterwas evaluated in comparison with poly(L-lactic acid) (PLA), poly (3-hydroxybutyrate)(PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) andpoly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Scanning electronmicroscopy (SEM) showed whorls-like morphology only on PHBVHHx film surface.Methylthiazol tetrazolium (MTT) assay demonstrated that PHBVHHx was better thanthe above four materials in promoting cell attachment and proliferation of fibroblastcell line L929 and osteoblast cell line MC3T3, respectively. Histological study usingrabbits proved that PHBVHHx was a fairly harmless implantable biomaterial. Thus,PHBVHHx with an adjustable mechanical property, combined with itsbiocompatibility, are in the process of developing into a new generation of bioimplantmaterial.