The Study Of Genetic Expression Of CD8B1 On The Peripheral Blood Of Aggressive Periodontitis

It has been suggested that Aggressive periodontitis(AgP) is one of main reasons that causes the healthy(?) young people to lost their teeth, which has a genetic basis and familial aggregation and develops rapidly. The classification of Aggressive periodontitis is ambiguity and the aetiology and pathogenesis of Aggressive periodontitis is unclear today as well. Not with standing, some microorganisms with special virulence significance factor that cause AgP, more and more evidence indicated that several factors associated with AgP. Presently, it is considered that the etiopathogenisis of AgP include predisposing factor of host, environmental factor and pathogenic microorganism and the predisposing factor of host is decided by related genes. It is indicated that Aggressive periodontitis may involve dysfunctions in the cellular immune responses by previous researchers. Previous researcher found out two groups genes (up-regulation and down-regulation) which level express differently in AgP group compared control by gene chip. We select CD8B1 which is associated with immunifaction closely from down-regulation group to do research to examine the result of gene chip then promote the research development of later stage.Objective: To detect the RNA transcriptional level and protein expression of CD8B1 and TPD52L2 which genes are express differently on peripheral blood in AgP by FQ-PCR and Western-blot. These can authenticate the results of gene chip and facilitate the later research on the etiopathogenisis and pathogenetic of AgP.Methods: Take suction 10ml blood of peripheral vein from every patient and healthy controls. The leucocytes were isolated from every sample. Using RT -PCR technique to detect the express level of the CD8B1 and TPD52L2 , then choose CD8Blwhich is low-express in AgP really to do western-blot.Result: Comparing with controls, the RNA transcriptional level and protein expression of CD8B1 all lower in AgP ; the RNA transcriptional level of TPD52L2 is higher, but the degree is not consistent with the result of gene chip.Conclusion: The differential expression of CD8B1 may be related to thePathogenesis of Aggressive periodontitis. CD8B1 express low level in AgP suggest that this gene may be associated with the genetic predisposition to aggressive periodontitis, but the number of sample is small and this result must to be made sure by using large samples.