| Object: To investigate the role of tertramethylpyrazine(TMP)in rats of reanl Ischemia Reperfusion Injury through the model, and study the possible mechanism.Methods: 72 male SD rats were randomized to the following three groups: Sham operation group(n=24), in this group, 24 rats were divide into four subgroups according to the time of harvesting sample, each subgroup has 6 rats;Ischemia-reperfusion group (n=24),in this group, 24 rats were divide into four subgroups according to the time of reperfusion, that is 1h, 6h, 12h, 24h after reperfusion, each subgroup has 6 rats, TMP treatment group(n=24), 24 rats were divide into four subgroups as ischemia- reperfusion group. Rats were anesthesized and undertook lapotomy. Sham operation group were treated without occlusion of left renal artery, only right-nephrectomized. In ischemia-reperfusion group and TMP treatment group, right nephrectomy was performed as soon as occlusion of left renal artery for 45 minutes and then recanalization. TMP were administered intravenously at a dose of 40mg/kg before occlusion of left renal artery , The rats in other two groups were IV normal saline equivalently. When the experiment was finished, left nephrectomy was performed in every group , at the same time , blood samples were obtained for measurement of serum levels of creatine(Cr) and blood urea nitrogen (BUN). On one hand, 0. 5g of every left kidney was obtained for measurement of tissue malondialdehyde(MDA) contents and superoxide dismutase(SOD) activety. On the other hand, the other tissue was used for histological examination, positive staining rate of tumor necrosis factorα(TNF-α) and interleukin-10(IL-10) was analyzed by immunohistochemical staining. Results: (1) Renal function change: Compared with Sham operation group, the serum levels of BUN in the subgroup of 1h rerperfusion in Ischemia-reperfusion group is not different statistically, and those in other subgroups were higher signifcantly in ischemia-reperfusion group (P<0.01) .The serum levels of Cr in ischemia-reperfusion group were significantly higher than those in Sham operation group (P<0.01). Compared with Sham operation group, the serum levels of BUN in the TMP treatment subgroups of 1h rerperfusion is not different statistically, and those in other subgroups were higher signifcantly in TMP treatment group (P<0.05, P<0.01). The serum levels of Cr in the each TMP treatment subgroup was higher signifcantly than those in corresponding sham operation subgroups (P<0.05, P<0.01). Compared with corresponding subgroups in ischemia-reperfusion group, the serum levels of BUN and Cr in the subgroups of 1h reperfusion were not different statistically, and those in other subgroups were lower signifcantly in TMP treatment group (P<0.05, P<0.01). (2)Tissue biochemical indicator in kidney: The levels of tissue MDA content and tissue SOD activity in ischemia-reperfusion group were significantly higher than those in the corresponding subgroups of Sham operation group (P<0.05,P<0.01). Compared with Sham operation group, Tissue SOD activity in kidney in the subgroup of 1h rerperfusion didn't decrease significantly, but that in other subgroups increased signifcantly in ischemia-reperfusion group(P<0.05,P<0.01). Compared with Sham operation group, the level of tissue MDA content in the subgroup of 24h rerperfusion in TMP treatment group was higher significantly than that in Sham operation group(P<0.01), but those in other subgroups were not different statistically than those in corresponding sham operation subgroups. Compared with corresponding sham operationsubgroups, tissue SOD activity in kidney in the TMP treatment subgroups of 1h rerperfusion didn't decrease significantly, but those in other subgroups decreased signifcantly in TMP treatment group(P<0.05). (3)the change of nephridial tissue histomorphology: Renal cellular damages in ischemia-reperfusion group showed more severe than those in Sham operation group. Compared with with Ischemia-reperfusion group, renal histologic injury were attenuated significantly by TMP treatment group. (4)positive staining rate of TNF-αand IL—10:compared with Sham operation group, the positive staining rate of TNF-αand IL-10 of ischemia-reperfusion group and TMP treatment group was higher significantly, compared with Ischemia-reperfusion group, the positive staining rate of TNF-αof TMP treatment group was signify-cantly decreased, but IL-10 was significantly increased. Conclusion:our results indicate that TMP can attenuate renal ischemia-reperfusion injury, which through against oxygen free radicals damage, shutdown the expression of TNF-αand promote the expression of IL-10.
|
PDF Full Text Request