Study On Serum Proteomics Of Mouse Hepatoma Cell Line H₂₂ And Treated By GaspGF

Objective: The technique and strategy of serum proteomics was used to differentiate and identify proteins secreted by mouse hepatoma cell line H₂₂ and normal mouse. Through the variation in serum protein expression map, we can observe the effectiveness of GASPGF.Construct technique platform on proteomics for research the traditional Chinese medicine inhibition tumor.Methods: support by electrophoretic technique, to set up the serum protein expression map of all groups that included group normal,group tumor and group GASPGF.Two-dimensional electrophoresis and one dimensional SDS-PAGE electrophorsis were performed to separate the proteins of every group, searched differentially expressed protein spots or straps.Results:â‘ The inhibition ratio of tumor is 48.92%.â‘¡one dimensional SDS-PAGE eletrophoresis analysis: group normal has 20 straps; group tumor has 24 straps; group GASPGF has 22 straps.â‘¢Use supernatant of serum-free medium of group normal,group tumor and group GASPGF to separated by Two-dimensional electrophoresis, we find that there are 51 protein spots in group normal, 55 protein spots in group tumor, 60 protein spots in group GSAPGF.Conclusion:â‘ Noticeable change before and after the tumor In serum protein expression map.The pathogenesis of transplantation tumor maybe concerned with regulation the proteins named Hsp,Gst,Cal,Arg,p21 and nmel.â‘¡Campared to group tumor, group GASPGF have notable difference on the inhibition ratio of tumorâ‘¢GASPGF can effect the serum proteomics of mouse, regulation the protein named Hsp,Cal,Arg,p21 and NMEL,which possiblily can be the mechanism of action of GASPGF to treat tumor.â‘£Construct technique platform on proteomics for research the traditional Chinese medicine inhibition tumor.