| ObjectiveOrganotin is one of the endocrine disruptors. The environmental pollution of organotin attracts more and more, because it's purpose is very widely and conduct toxicy to organism by very lower dosage (10-12). DBTD primarily used to stabilize polyvinyl chloride (PVC), defend the dirty coating, as insecticide and fungicidal etc. The health effects of DBTD are irritating to skin and mucous membrane, and its effects on digestive, nerval, immune ,endocrine and reproductive systems. In order to elucidate the effects of DBTD on liver toxicity, the change of biochemical indexes in serum and liver were measured, DNA damage and apoptosis in liver were also observed, and then explore the toxicity of DBTD on liver.MethodsChoose 40 Healthy male rats from Animal Center of JiLin University and grouped 4 groups. Body weight of the rat was 190±10 g.There were 10 rats in each group. And they were exposed to DBTD by using the intragastric administration for 8w at the dose of 0, 5, 10 and 20 mg/kg respectively. ALL rats of each group were sacrificed on the 48h after last exposure to DBTD.To take the blood from the femoral of rats and put to death. Use reagent to detect the AST, ALT, GGT, ALP and TBA activity of serum.To take the liver from the body of rats and put to death.,The level of MDA and the activity of SOD and GSH-PX of hepatic homogenate were determined.Put the liver organization of surplus immediately into 10% of neuter gender in the formaldehyde aqua fix, ether steps degree dehydrate, normal regulations paraffin wax pack cover up, interrupted even slice 5～10, slice thickness 5μm, Su the wood Jing-Yi be red dye, light the mirror descend observation appearance to learn variety.DNA damage was measured by the single cell gel electrophoresis(SCGE), observed cell with the fluorescence microscope descend an observation, the each slice count 100 cell and computing a total DNA damage rate.Cell cycle were detected with flow cytometry.Statistics analyses of quantitative data were conducted usingvariance analyses .Categorical data were analyzed by Chi-square test.Result1, Effect of DBTD on the body weight of male rat: The body weight increased in lower and medium dosage group while the weight of body dexreased in high dosage group in the 6～8 week, compared with control group P<0.05.2, Effect of DBTD on enzyme activities of male rat in serum: The activities of enzyme ALT increased with the dose of DBTD in each exposure group, and compared with control group and each exposure group P<0.05. The content of AST in serum have the trend of increased with the increased of dose, medium and high dosage group have significantly differently with the control group and lower dosage group P<0.05. GGT and TBA content in the serum was obvious high in matched high dosage groups, compared with the control, lower, medium dosage groups P<0.05. The content of ALP increased with the dose of DBTD in each exposure group, and high dosage group compared with control group and lower dosage group P<0.05.3, Effect of DBTD on biochemical indexes of male rats in liver: The content of MDA in liver have the trend of increased with the dose of DBTD in each exposure group, and medium, high dosage group compared with control group P< 0.05. The content of SOD and GSH-PX in liver have the trend of decreased in each exposure group, and high dosage group have significantly with control group and lower group P<0.05.4, Pathologic changes of liver in rat: The result of pathological sections showed that the control and lower group havn't seen difference in structure. Lobule biliary duct in medium dosage group have signicantly meaning and compared with control group, and biliary duct epithelioid cell was a little hyperplasia,and duct has a light dilatation. In the high dosage group, it can seen increased of lobule biliary duct, biliart epithelioid cell hyperplasia, and duct has a little dilatation.5, Change of DBTD on DNA damage and the apoptosis. The ratio of DNA damage on liver of rats significantly increase in lower, medium and high dosage group. And the lower group compared with control group P<0.05, the medium ,high dosage group compared with control group have significantly meaning P<0.01.The result of statistic on apoptosis, with the increase of dose, G0/G1 period have obvious descread, S and G2/M period have clear increased.with control group.The period of G0/G1 and S have significantly difference in medium and high dosage group compared with control and lower group P< 0.05. The period of G2/M in medium dosage group compared with control group P<0.05, high dosage group have clear difference compared with control and lower group P<0.05.Conclusion Result from the present reseach showed that long-term exposure to DBTD can cause pathologic changes of capillary bile duct from male rats,increasing activities of enxymes of ALT,AST,GGT,ALT and TBA in serum . The content of MDA in liver of rats were increased. The content of SOD and GSH-PX in liver of rats were decreased. The total damage of DNA in liver cell of rats had obvious increased. The period of G0/G1 was increased in cell circle. DBTD have toxicity to liver of rats.
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