Isolation Of Antiangiogenesis Peptide From Coina Intestinalis (PCI) And Study Of Its Antiangiogenesis Activity

Angiogenesis is the essential pathway of the tumor growth and metastasis, and have compact relation with the arision and diversion of solide tumor. Many tumors occour clinic symptom only after new blood vessels formade. Antiangiogenic therapy is thought to be free of the severe side dffects and being a breakthrough of the last severaldecades. Coina intestinalis is the major material of nevers'development reseach and immune system evolution, and was translated the genome at 2002. Many anticancer peptides were pured in other class of tunicate without reports about peptides with antiangenesis activity from Coina intestinalis. This paper got a peptide using many isolated metherds and did some research about its antiangensis activeity.Angiogenesis peptide (PCI) was isolated from the Coina intestinalis. The Coina intestinalis were deposited with 70% acetone water solution following by gel chromatography of SephadexG25. After the Superdex75 FPLC assay, the peptides BLF-2 ware obtained. Cell proliferation assay in vitro, using MTT method showed that the concentrations of BLF-2 required for 50% growth inhibition of HUVECs was 8μg/ml. Zebra fish angiogenesis model in vivo, showed the BLF-2 inhibited the growth of SIV of zebrafish embryos significantly in a dose-dependent manner and SIV of zebrafish embryos treated with 40μg/ml BLF-2 for 12h was observed almost complete inhibiton.