| Renal cell carcinoma (RCC) represents the most common cancer of kidney and accounts for approximately 3% of all adult malignancies in western countries, and due to its insidious onset patients frequently have advanced disease at the time of clinical presentation. Proteins are the key materials to understand cellular functions and disease process, such as tumorigenesis, tumor growth and metastases. Thus, it is expected that protein expression would differ between normal kidney tissue and homologous RCC tissue. The technique proteome-based has been shown to be a useful tool to identify novel proteins for diagnosis and prognosis of malignant disease including colorectal carcinoma , lung , and breast cancer . In this study, we prepared proteins from the RCC tissue and the homologous normal kidney tissue, and analyzed a number of proteins to isolate and identify tumor-specific proteins of RCC by two-dimensional gel electrophoresis.Objective: To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from renal cell carcinoma (RCC)tissue and paired normal tumor-adjacent kidney tissue, and to identify the differential expression proteins.Methods: The total proteins of RCC tissue examined by histological and paired normal tumor-adjacent kidney tissue were separated by two-dimensional gel electrophoresis(2-DE),analyzed by using Image Master 2D software, and identified by peptide mass fingerprint(PMF)and database searching .Results: Well-resolved, reproducible 2-D maps of above tissues were obtained. Some of different proteins identified by mass spectrometry(MS) were matched in SWISS-2DPAGE database.Conclusion: We established the expression maps of proteins and analyzed the significant differences among RCC and normal kidney tissues. These data would be valuable for studying the diagnostic markers, mechanisms of metastasize, therapeutic targets and so on. |
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