On Expression Of VEGF-C And VEGFR3 In Breast Carcinoma Tissue And In Peritumoral Tissue And Their Relations With Lymph Metastasis

Lymph metastasis is the main channel of early metastasis of breast cancer. Transfer of cancer cells to lymph nodes forms a metastasis area and may damage local tissues, and may hide in lymph nodes, and may develop into a source for further transfer to other organs. Any lymph metastasis may give negative impact to the treatment. Therefore, a study on related factors of lymph metastasis of breast cancer is of great significance to effective containment of farther transfer of cancer cell, to judgement on the treatment, and choice of treatment plans.In this study, immunohistochemistry SP method was used to examine the expression of VEGF-C and VEGFR3 in 70 cases of breast cancer and in it's peritumoral tissue, while testing the expression of CK19 in lymph node to explore lymph metastasis. The purpose of this program is to study the expression, distribution and location of VEGF-C and VEGFR3 in breast carcinoma tissue and in peritumoral tissue; to discuss the contribution and its mechanism of VEGF-C and VEGFR3 to the newly borned lymphatic vessels in the breast cancer; to explore the role and its mechanism of the newly borned lymphatic vessels in lymph metastasis of breast cancer; and to provide new effective methods for clinical judgment on whether lymph metastasis occurs in breast cancer cases.Results of this study runs as follows: 117 lymph nodes in32 cases positive and 38 negative cases of The lymph node metastasis are checked out in all 70 cases of breast cancer .The positive expression rate of VEGF-C in breast carcinoma tissue is 78.6 % (55/70) , and its rate in peritumoral tissue is 54.3 % (38/70) .There are significant ststistic difference between the two groups(P<0.01). In peritumoral tissue the positive lymph vessel of VEGFR3 is higher(10.306 ±6.158)than its in breast carcinoma tissue(7.896±5.565) (P<0.01 ).The expression of VEGFR3 in peritumoral tissue is closely correlated with VEGF-C expressed in breast carcinoma tissue (γ= 0.66, P<0.05) . With the increase of the expression of VEGF-C in breast carcinoma tissue, the rate of VEGFR3 positive vessels is increasing. In breast cancer, the lymph vessel of VEGFR3 positive is located in lymphatic vascular endothelial cells of tumorstroma tissue and peritumoral tissue, and mature lymphatic vessels are not found in cancer nest. In breast carcinoma tissue the positive expression rate of VEGF-C in lymph node metastasis group(87.5%) is significantly higher than that of no lymph node metastasis group(71.1 %) (P<0.01) . In peritumoral tissue, the number of VEGFR3 positive vessels in lymph node metastasis group (12.484±6.505) is significantly higher than in non-lymph node metastasis group(8.471±6.017)( P<0.01 ).The density of newly lymph vessels in breast cancer is related with the size of the tumor and the clinical stage of tumor and the expression of CerbB-2, but has no relation with pathological type of tumor and the expression of ER and PR, as well as the age of patients.Conclusion: The positive expression of VEGF-C is located in breast cancer cells, and VEGFR3, the receptors of VEGF-C is located in lymphatic vascular endothelial cells of tumor stroma tissue and peritumoral tissue, and mature lymphatic vessels are not found in cancer nest. The expression of VEGFR3 in peritumoral tissue is positively related with VEGF-C expressed in breast carcinoma tissue. VEGF-C and VEGFR3 are closely correlated to the lymphangiogenesis and lymph node metastasis of breast cancer.