The Experimental Study Of Aresenic Trioxide For Increasing Radiosensitivity In ASPC-1 Human Pancreatic Carcinoma Cell Lines

Objective:To evaluate the radiosensitive effect of Arsenic trioxide(AS2O3) on the ASPC-1 human pancreatic carcinoma cell line .Methods:The methods of MTT were used to analysis the cell cytotoxics in the ASPC-1 cell line with AS2O3 concentration of 0umol/l,2.5 umol/l,5 umol/l,10 umol/l,20 umol/l,40 umol/l and 80umol/l,and the concentration needed to inhibit 50% of cell survival(ID50) was calculated.The methods of MTT were used to analysis the cell survival rates in the different groups of ASPC-1 cell line with AS2O3 concentration of 0umol/l,2.5 umol/l,5 umol/l,and 10umol/l combined with irradiated of 2Gy,5Gy and 10Gy within 48 hours after incubation with AS2O3 and in the groups of ASPC-1 cell lines with irradiation alone.The cell survival curves were determined for evaluating the cell killing effects.Flow cytometry was performed to access the influence of AS2O3 on cell cycle distribution.Results:Detection of MTT colorimetric assay:48 hours later , the inhibition ratio of different concentrations(2.5,5.0,10,20,40,80 umol/l) of AS2O3 are 2.32%,5.14%,11.01%,29.42%,53.84%,80.21%,and 50% inhibitory dose (ID50) was 34.91umol/l. it was shown that the intensities of cell proliferating inhibition seened to be much dependent on increasing of AS2O3 concentration .The cell survival rates were lower in the combination groups than those in the groups ofirradiation alone.Cell suevial cueves showed that the inhibitory effect was much more evident when AS2O3 at 5umol/L and 5Gy were applied combinatively than either one of them was used alone.Flow cytometric analysis indicated that AS2O3 induced G2/M phase arrest obviously in the cell cycle of the ASPC-1 cell lines 48houres after irradiation exposure.Conclusion:It was concluded that AS2O3 might be a potential radiosensitizer for the further study of the ASPC-1 human pancreatic carcinoma cell line.