The Study On Relationship Between PIGF And Gastric Adenocarcinoma Angiogenesis

Background and objective:The gastric cancer is common tumor with a high death rate about 20.93/100,000. The invasiveness proliferation and the metastasis of the tumor both rely on the angiogenesis of tumor, Angiogenesis is a hot spot of the cancer research because of the significant effect on invasiveness proliferation and metastasis of tumor. The previous study showed that VEGF (Vascular endothelial growth factor) plays a key role during the growth of the tumor. While the PIGF(placenta growth factor), as a new member of the VEGFs, was expressed in many tumors. Few researches on PIGF in gastric cancer were reported although the functions of the VEGF have been studied many years. In this paper, the study focuses on the expression of the VEGF and PIGF in tissues of gastric cancer and para-tumor. The results show that PIGE has an important significance in directing the anti-angiogenesis therapy and raising the survival rates.Materials and methods1. Materials:96 samples of the gastric adenocarcinoma come from the General Hospital of Shenyang Military Command during 2004 to 2006 with the age from 23 to 72, are 57 male and 39 female . The section are recut for 4μm section and stained with routine HE .2.Methods:Immunohistochemistry: polyclonal anti PIGF was purchased from SantaCruz,monoclonal antibody of CD34,VEGF were purchased from Shanghai changdao Company , Streptavidin / Peroxidase(SP) perform according to standard protocol except that the primary antibody wereCD34,VEGF,P1GF. Immunohistochemistry was performed according to protocol of ABC Kit.3. Evolution3.1 Evolution of the MVD: The microvessel was labeled by CD34. The stained capillary vessel and microvessel were accounted according to the standard of the Weidner [1]. Any individual brown endothelial cell and cell cluster were account as one while the area of the hemocoele higher than 8 times of diameter of the red cells should be ignored. The methods of count: Count the CD34 positive microvessels under 200×. Four different views were selected random for counting the microvessels. The mean value is the MVD ( number/x200)3.2 Evaluation of VEGF and PIGF:Positive VEGF expression defined as brown particles existed in cytoplasm and membrane.3.3 Statistical analysis:All statistical analyses were performed using the Statistical Package for SPSS ll.5. The association of variables was evaluated using theχ~2 test,t-test and Spearman analysis.ResultsVEGF was expressed in 47.92 %(46/96) of the gastric carcinoma. The MVD of VEGF positive gastric carcinoma was 31.4±8.5/oil lens, and 19.22±8.2/ oil lens in VEGF negative tissues. It has significant difference (P<0.01). The MVD of PIGF positive intestines carcinoma was 34.1±1.4/ oil lens and 30.8±2.6/ oil lens in PIGF negative tissues. It has significant difference (P<0.01). The MVD of PIGF,VEGF in gastric carcinoma is significantly higher than normal mucous (P<0.05). The MVD of PIGF,VEGF is significant association with gastric carcinoma Duke's stages and the translocation of lymph. (P<0.05). The immunohistochemistry results showed that the expression of VEGF in gastric carcinoma was significant difference with para-tumor (P<0.05). The MVD in low-grade gastric carcinoma was significant higher than mid-grade and high-grade type (P<0.05), but there is no significant difference between mid-grade and high-grade gastric carcinoma (P>0.05). The association of variables analysis showed that VEGF expression and the MVD value was significantly positive correlation (r=0.541,P<0.01),while PIGF expression and MVD value was also significantly positive correlation(r=0.392 ,P<0.01).Conclusion:1. The expression of VEGF in three type gastric carcinoma and para-tumor was significant difference. The expression of VEGF in poorly differentiated adenocarcinoma and middle differentiated, highly differe- ntiated adenocarcinoma was different.2. The expression of PIGF in three type gastric carcinoma and para-tumor, was significant different.The expression of PIGF in poorly differentiated adenocarcinoma and middle differentiated, highly differen- tiated adenocarcinoma was different.3. VEGF and PIGF are significantly positive coordinated to angiogenesis in gastric carcinoma, especially in poorly differentiated gastric carcinoma. This provides a new methods using vascular endothelial growth inhibitor to theat gastric carcinoma and raise the survival rate.